| Title: |
Combined point-of-care nucleic acid and antibody testing for SARS-CoV-2 following emergence of D614G spike variant |
| Authors: |
Mlcochova, P; Collier, D; Ritchie, A; Assennato, SM; Hosmillo, M; Goel, N; Meng, B; Chatterjee, K; Mendoza, V; Temperton, N; Kiss, L; James, LC; Ciazynska, KA; Xiong, X; Briggs, JAG; Nathan, JA; Mescia, F; Bergamaschi, L; Zhang, H; Barmpounakis, P; Demeris, N; Skells, R; Lyons, PA; Bradley, J; Baker, S; Allain, JP; Smith, KGC; Bousfield, R; Wilson, M; Sparkes, D; Amoroso, G; Gkrania-Klotsas, E; Hardwick, S; Boyle, A; Goodfellow, I; Gupta, RK; Dougan, G; Gupta, R; Lehner, PJ; Lyons, P; Matheson, NJ; Toshner, M; Weekes, MP; Brown, N; Curran, M; Palmar, S; Enoch, D; Chapman, D; Shaw, A |
| Publisher Information: |
Cell Press |
| Publication Year: |
2021 |
| Collection: |
Oxford University Research Archive (ORA) |
| Description: |
Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.1016/j.xcrm.2020.100099 |
| DOI: |
10.1016/j.xcrm.2020.100099 |
| Availability: |
https://doi.org/10.1016/j.xcrm.2020.100099; https://ora.ox.ac.uk/objects/uuid:739e6ad4-1fa2-4291-8d22-43d09802ab62 |
| Rights: |
info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| Accession Number: |
edsbas.DDE4FB93 |
| Database: |
BASE |