| Title: |
Aerobic Conditions and Endogenous Reactive Oxygen Species Reduce the Production of Infectious MS2 Phage by Escherichia coli |
| Authors: |
Bastin, Guillaume; Galmiche, Aurélie; Talfournier, François; Mazon, Hortense; Challant, Julie; Robin, Maëlle; Majou, Didier; Boudaud, Nicolas; Gantzer, Christophe |
| Contributors: |
Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME); Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS); Actalia Saint-Lô; Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA); Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS); Association de Coordination Technique Agricole pour l'Industrie Agro-Alimentaire (ACTIA); ANR-15-IDEX-0004,LUE,Isite LUE(2015) |
| Source: |
ISSN: 1999-4915 ; Viruses ; https://hal.science/hal-03338456 ; Viruses, 2021, Phage-Host Interactions 2021, 13 (7), pp.1376. ⟨10.3390/v13071376⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2021 |
| Collection: |
Université de Lorraine: HAL |
| Subject Terms: |
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Description: |
International audience ; Most of the defective/non-infectious enteric phages and viruses that end up in wastewater originate in human feces. Some of the causes of this high level of inactivity at the host stage are unknown. There is a significant gap between how enteric phages are environmentally transmitted and how we might design molecular tools that would only detect infectious ones. Thus, there is a need to explain the low proportion of infectious viral particles once replicated. By analyzing lysis plaque content, we were able to confirm that, under aerobic conditions, Escherichia coli produce low numbers of infectious MS2 phages (I) than the total number of phages indicated by the genome copies (G) with an I/G ratio of around 2%. Anaerobic conditions of replication and ROS inhibition increase the I/G ratio to 8 and 25%, respectively. These data cannot only be explained by variations in the total numbers of MS2 phages produced or in the metabolism of E. coli. We therefore suggest that oxidative damage impacts the molecular replication and assembly of MS2 phages. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34372580; PUBMED: 34372580; PUBMEDCENTRAL: PMC8310082 |
| DOI: |
10.3390/v13071376 |
| Availability: |
https://hal.science/hal-03338456; https://hal.science/hal-03338456v1/document; https://hal.science/hal-03338456v1/file/viruses-13-01376-v2.pdf; https://doi.org/10.3390/v13071376 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.DFF50451 |
| Database: |
BASE |