| Title: |
An omicron-specific neutralizing antibody test predicts neutralizing activity against XBB 1.5 |
| Authors: |
Stephen Varvel; Marzena Galdzicka; Stephanie Nystrom; Hong Liu; Ge Chen; Izabela Ragan; Shahrokh Shabahang |
| Source: |
Frontiers in Immunology, Vol 15 (2024) |
| Publisher Information: |
Frontiers Media S.A. |
| Publication Year: |
2024 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
SARS-CoV-2; omicron; neutralizing antibody; flow cytometry; XBB.1.5; Immunologic diseases. Allergy; RC581-607 |
| Description: |
IntroductionUnderstanding the immune status of an individual using neutralizing antibody testing is complicated by the continued evolution of the SARS-CoV-2 virus. Previous work showed that assays developed against the wildtype strain of SARS-CoV-2 were insufficient predictors of neutralization of omicron variants, thus we developed an omicron-specific flow cytometry-based neutralizing antibody test and performed experiments to assess how well it compared to an omicron-specific PRNT assay (gold standard) and whether it could predict neutralizing activity to more recent omicron subvariants such as XBB.1.5.MethodsAccuracy of a novel flow cytometry-based neutralizing antibody (FC-NAb) assay was determined by comparison with an omicron-specific PRNT assay. A series of samples were evaluated in both the omicron FC-NAb assay and a second test was designed to assess neutralization of XBB.1.5.ResultsGood concordance between the omicron FC-NAb test and the omicron PRNT was demonstrated (AUC = 0.97, p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fimmu.2024.1334250/full; https://doaj.org/toc/1664-3224; https://doaj.org/article/d1587a3c4fbc456789a569a43de2afc7 |
| DOI: |
10.3389/fimmu.2024.1334250 |
| Availability: |
https://doi.org/10.3389/fimmu.2024.1334250; https://doaj.org/article/d1587a3c4fbc456789a569a43de2afc7 |
| Accession Number: |
edsbas.E1660FF5 |
| Database: |
BASE |