| Title: |
Rice Yellow Mottle Virus resistance by genome editing of the Oryza sativa L. ssp. japonica nucleoporin gene OsCPR5.1 but not OsCPR5.2 |
| Authors: |
Arra, Yugander; Auguy, Florence; Stiebner, Melissa; Chéron, Sophie; Wudick, Michael, M; Miras, Manuel; Schepler‐luu, Van; Köhler, Steffen; Cunnac, Sebastien; Frommer, Wolf, B; Albar, Laurence |
| Contributors: |
Heinrich Heine Universität Düsseldorf = Heinrich Heine University Düsseldorf; Plant Health Institute of Montpellier (UMR PHIM); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM); Nagoya University; Alexander von Humboldt Professorship (WF), the Bill and MelindaGates Foundation to Heinrich Heine University, D€usseldorf, with asubaward to the University of Missouri (BY) (OPP1155704); Deutsche Forschungsgemeinschaft (DFG, German ResearchFoundation) under Germany’s Excellence Strategy–EXC-2048/1–project ID 390686111 (CEPLAS); Marie Skłodowska-Curiefellowship under the European Union’s Horizon 2020 research and innovation programme (“PDgate” No. 101023981); fellowship by the Alexander von HumboldtFoundation; CGIAR ResearchProgram on rice agri-food systems (RICE, 2017–2022). |
| Source: |
ISSN: 1467-7644. |
| Publisher Information: |
CCSD; Wiley |
| Publication Year: |
2024 |
| Collection: |
Université de Montpellier: HAL |
| Subject Terms: |
genome editing; CRISPR/Cas9; knockout mutant; disease resistance; recessive; nucleoporin; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN]; [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy |
| Description: |
International audience ; Rice yellow mottle virus (RYMV) causes one of the most devastating rice diseases in Africa. Management of RYMV is challenging. Genetic resistance provides the most effective and environment-friendly control. The recessive resistance locus rymv2 (OsCPR5.1) had been identified in African rice (Oryza glaberrima), however, introgression into Oryza sativa ssp. japonica and indica remains challenging due to crossing barriers. Here, we evaluated whether CRISPR/Cas9 genome editing of the two rice nucleoporin paralogs OsCPR5.1 (RYMV2) and OsCPR5.2 can be used to introduce RYMV resistance into the japonica variety Kitaake. Both paralogs had been shown to complement the defects of the Arabidopsis atcpr5 mutant, indicating partial redundancy. Despite striking sequence and structural similarities between the two paralogs, only oscpr5.1 loss-of-function mutants were fully resistant, while loss-of-function oscpr5.2 mutants remained susceptible, intimating that OsCPR5.1 plays a specific role in RYMV susceptibility. Notably, edited lines with short in-frame deletions or replacements in the N-terminal domain (predicted to be unstructured) of OsCPR5.1 were hypersusceptible to RYMV. In contrast to mutations in the single Arabidopsis AtCPR5 gene, which caused severely dwarfed plants, oscpr5.1 and oscpr5.2 single and double knockout mutants showed neither substantial growth defects nor symptoms indicative lesion mimic phenotypes, possibly reflecting functional differentiation. The specific editing of OsCPR5.1, while maintaining OsCPR5.2 activity, provides a promising strategy for generating RYMV-resistance in elite Oryza sativa lines as well as for effective stacking with other RYMV resistance genes or other traits. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
IRD: fdi:010088843; WOS: 001130111500001 |
| DOI: |
10.1111/pbi.14266 |
| Availability: |
https://hal.inrae.fr/hal-04531325; https://hal.inrae.fr/hal-04531325v1/document; https://hal.inrae.fr/hal-04531325v1/file/Arra-PJB-2024-CC-BY-NC-ND.pdf; https://doi.org/10.1111/pbi.14266 |
| Rights: |
http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.E1F0BA57 |
| Database: |
BASE |