| Title: |
Identification and Ultrastructural Peculiarities of Nestin-Carrying Progenitor Cells in Kidney |
| Authors: |
Valeriya B. Vays; Irina M. Vangeli; Lora E. Bakeeva; Ciara I. Makievskaya; Vasily A. Popkov; Ljubava D. Zorova; Igor I. Kireev; Savva D. Zorov; Nadezda V. Andrianova; Marina I. Buyan; Valentina A. Babenko; Anna V. Tvorogova; Egor Y. Plotnikov; Genady T. Sukhikh; Dmitry B. Zorov |
| Source: |
International Journal of Molecular Sciences ; Volume 27 ; Issue 4 ; Pages: 1695 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2026 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
nestin; stem cells; ultrastructure; mitochondrial membrane potential; vesicles; segmentation |
| Description: |
In this study, in a culture of renal epithelial cells, we identified those expressing nestin, a cytoskeletal protein associated with stem/progenitor/activated/proliferating cell states. A mouse expressing GFP under the nestin promoter was used, followed by cell isolation and culture. It is hypothesized that this can be used to assess the stem/progenitor/activated/proliferating cell level in a mixed kidney cell culture. Both nestin-positive and nestin-negative cells were demonstrated to be present in the culture. After visualization, cells were attached to a glass slide with a grid, fixed, and prepared for electron microscopy analysis, with each cell visually identified by light microscopy being analyzed. Electron microscopy revealed tight interactions between nestin-positive and nestin-negative cells. Significant differences in the ultrastructure of nestin-positive and nestin-negative cells were observed. Nestin-positive cells were distinguished by a high ribosome content, indicating high protein-synthesizing activity. In the nestin–GFP-high (sorted) population examined by electron microscopy, vesicle-containing protrusions were frequently observed. These cells could contain multiple nuclei of varying sizes and had a high content of lysosomes. No significant differences in mitochondrial ultrastructure were observed in nestin-positive and -negative cells, although functional characteristics evaluated by the membrane potential probe differed. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Biochemistry; https://dx.doi.org/10.3390/ijms27041695 |
| DOI: |
10.3390/ijms27041695 |
| Availability: |
https://doi.org/10.3390/ijms27041695 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.E25EF46 |
| Database: |
BASE |