| Title: |
Antimicrobial effects of silver nanoparticle-microspots on the mechanical properties of single bacteria |
| Authors: |
Caniglia, Giada; Valavanis, Dimitrios; Tezcan, Gözde; Magiera, Joshua; Barth, Holger; Bansmann, Joachim; Kranz, Christine; Unwin, Patrick R. |
| Publisher Information: |
Royal Society of Chemistry |
| Publication Year: |
2024 |
| Collection: |
The University of Warwick: WRAP - Warwick Research Archive Portal |
| Subject Terms: |
QD Chemistry; QH Natural history |
| Description: |
Silver nanoparticles (AgNPs) conjugated with polymers are well-known for their powerful and effective antimicrobial properties. In particular, the incorporation of AgNPs in biocompatible catecholamine-based polymers, such as polydopamine (PDA), has recently shown promising antimicrobial activity, due to the synergistic effects of the AgNPs, silver(I) ions released and PDA. In this study, we generated AgNPs-PDA-patterned surfaces by localised electrochemical depositions, using a double potentiostatic method via scanning electrochemical cell microscopy (SECCM). This technique enabled the assessment of a wide parameter space in a high-throughput manner. The optimised electrodeposition process resulted in stable and homogeneously distributed AgNP-microspots, and their antimicrobial activity against Escherichia coli was assessed using atomic force microscopy (AFM)-based force spectroscopy, in terms of bacterial adhesion and cell elasticity. We observed that the bacterial outer membrane underwent significant structural changes, when in close proximity to the AgNPs, namely increased hydrophilicity and stiffness loss. The spatially varied antimicrobial effect found experimentally was rationalised by numerical simulations of silver(I) concentration profiles. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
0003-2654 |
| Relation: |
https://wrap.warwick.ac.uk/id/eprint/189660/1/d4an00174e.pdf; Caniglia, Giada, Valavanis, Dimitrios, Tezcan, Gözde, Magiera, Joshua, Barth, Holger, Bansmann, Joachim, Kranz, Christine and Unwin, Patrick R. (2024) Antimicrobial effects of silver nanoparticle-microspots on the mechanical properties of single bacteria. The Analyst, 149 (9). pp. 2637-2646. doi:10.1039/D4AN00174E ISSN 0003-2654. |
| DOI: |
10.1039/D4AN00174E |
| Availability: |
https://wrap.warwick.ac.uk/id/eprint/189660/; https://wrap.warwick.ac.uk/id/eprint/189660/1/d4an00174e.pdf; https://doi.org/10.1039/D4AN00174E |
| Rights: |
cc_by |
| Accession Number: |
edsbas.E35B965B |
| Database: |
BASE |