| Title: |
Amino Acid-Based Protein-Mimic Hydrogel Incorporating Pro-Regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds |
| Authors: |
Lu, Yan; Su, Shanchun; Chu, Chih Chang; Kobayashi, Yuichi; Masoud, Abdul Razak; Peng, Hongying; Lien, Nathan; He, Mingyu; Vuong, Christopher; Tran, Ryan; Hong, Song |
| Source: |
School of Medicine Faculty Publications |
| Publisher Information: |
LSU Health Digital Scholar |
| Publication Year: |
2024 |
| Subject Terms: |
14S; 21R-dihydroxy-docosa-4Z; 7Z; 10Z; 12E; 16Z; 19Z-hexaenoic acid; or 14S; 21R-diHDHA; 3° burn: third-degree burn; AA-PEA: amino acid-based poly(ester amide) polymer; ACgel1; UArg-PEA/Chitosan covalently coupled hydrogel 1; blood vessel regeneration or vascularization; collagen; MVFs: microvascular fragments; PreM1-MVFs-ACgel1: PreM1-incorporated and MVFs-seeded ACgel1; PreM1: pro-regenerative lipid mediator 1; UArg-PEA: unsaturated arginine-based poly(ester amide); wound healing; Biomedical Engineering and Bioengineering; Environmental Health and Protection; Neurosciences |
| Description: |
Pro-regenerative lipid mediator 1 (PreM1) is a specialized pro-resolving lipid mediator that promotes wound healing and regenerative functions of mesenchymal stem cells (MSCs), endothelial cells, and macrophages. The healing of third-degree (3°) burns and regenerative functions of MSCs are enhanced by ACgel1, an arginine-and-chitosan-based protein-mimic hybrid hydrogel. Adipose-tissue derived microvascular fragments (MVFs) are native vascularization units and a rich source of MSCs, endothelial cells, and perivascular cells for tissue regeneration. Here we describe an innovative PreM1-MVFs-ACgel1 construct that incorporated PreM1 and MVFs into ACgel1 via optimal design and fabrication. This construct delivered PreM1 to 3°-burn wounds at least up to 7 days-post-burn (dpb), and scaffolded and delivered MVFs. PreM1-MVFs-ACgel1 promoted the healing of 3°-burns in mice, including vascularization and collagen formation. The re-epithelization and closure of 3° burn wounds were promoted by ACgel1, MVFs, PreM1, MVFs-ACgel1, PreM1-ACgel1, or PreM1-MVFs-ACgel1 at certain time-point(s), while PreM1-MVFs-ACgel1 was most effective with 97% closure and 4.69% relative epithelial gap at 13 dpb compared to saline control. The PreM1-ACgel1 and MVFs-ACgel1 also promoted blood vessel regeneration of 3°-burns although PreM1-MVFs-ACgel1 is significantly more effective. These PreM1- and/or MVF-functionalized ACgel1 have nonexistent or minimal graft-donor requirements and are promising adjuvant therapeutic candidates for treating deep burns. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
https://digitalscholar.lsuhsc.edu/som_facpubs/3003; https://digitalscholar.lsuhsc.edu/context/som_facpubs/article/4005/viewcontent/AminoAcidBasedProteinMimic_2024.pdf |
| DOI: |
10.3390/ijms251910378 |
| Availability: |
https://digitalscholar.lsuhsc.edu/som_facpubs/3003; https://doi.org/10.3390/ijms251910378; https://digitalscholar.lsuhsc.edu/context/som_facpubs/article/4005/viewcontent/AminoAcidBasedProteinMimic_2024.pdf |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.E47FA4B1 |
| Database: |
BASE |