| Title: |
Depth distributions of nitrite reductase (nirK) gene variants reveal spatial dynamics of thaumarchaeal ecotype populations in coastal Monterey Bay |
| Authors: |
Reji, Linta; Tolar, Bradley B.; Smith, Jason M.; Chavez, Francisco P.; Francis, Christopher A. |
| Contributors: |
National Science Foundation; Achievement Rewards for College Scientists Foundation; U.S. Department of Energy |
| Source: |
Environmental Microbiology ; volume 21, issue 11, page 4032-4045 ; ISSN 1462-2912 1462-2920 |
| Publisher Information: |
Wiley |
| Publication Year: |
2019 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Summary Ammonia‐oxidizing archaea (AOA) of the phylum Thaumarchaeota are key players in nutrient cycling, yet large gaps remain in our understanding of their ecology and metabolism. Despite multiple lines of evidence pointing to a central role for copper‐containing nitrite reductase (NirK) in AOA metabolism, the thaumarchaeal nirK gene is rarely studied in the environment. In this study, we examine the diversity of nirK in the marine pelagic environment, in light of previously described ecological patterns of pelagic thaumarchaeal populations. Phylogenetic analyses show that nirK better resolves diversification patterns of marine Thaumarchaeota , compared to the conventionally used marker gene amoA . Specifically, we demonstrate that the three major phylogenetic clusters of marine nirK correspond to the three ‘ecotype’ populations of pelagic Thaumarchaeota . In this context, we further examine the relative distributions of the three variant groups in metagenomes and metatranscriptomes representing two depth profiles in coastal Monterey Bay. Our results reveal that nirK effectively tracks the dynamics of thaumarchaeal ecotype populations, particularly finer‐scale diversification patterns within major lineages. We also find evidence for multiple copies of nirK per genome in a fraction of thaumarchaeal cells in the water column, which must be taken into account when using it as a molecular marker. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/1462-2920.14753 |
| Availability: |
https://doi.org/10.1111/1462-2920.14753; https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.14753; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.14753; https://sfamjournals.onlinelibrary.wiley.com/doi/am-pdf/10.1111/1462-2920.14753 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.E6A4F1ED |
| Database: |
BASE |