| Title: |
Central-pair-linked regulation of microtubule sliding by calcium in flagellar axonemes |
| Authors: |
Nakano, Izumi; Kobayashi, Takeshi; Yoshimura, Misako; Shingyoji, Chikako |
| Publisher Information: |
Company of Biologists |
| Publication Year: |
2003 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Research Article |
| Description: |
The movement of eukaryotic flagella and cilia is regulated by intracellular calcium. We have tested a model in which the central pair of microtubules mediate the effect of Ca2+ to modify the dynein activity. We used a novel microtubule sliding assay that allowed us to test the effect of Ca2+ in the presence or absence of the central-pair microtubules. When flagellar axonemes of sea-urchin sperm were exposed to ATP in the presence of elastase, they showed different types of sliding disintegration depending on the ATP concentration: at low concentrations of ATP (≤50 μM), all the axonemes were disintegrated into individual doublets by microtubule sliding; by contrast, at high ATP concentrations (≥100 μM), a large proportion of the axonemes showed limited sliding and split lengthwise into a pair of two microtubule bundles, one of which was thicker than the other. The sliding behaviour of the axonemes was also influenced by Ca2+. Thus, at 1 mM ATP, the proportion of axonemes that split into two bundles increased from 25% at |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://jcs.biologists.org/cgi/content/short/116/8/1627; http://dx.doi.org/10.1242/jcs.00336 |
| DOI: |
10.1242/jcs.00336 |
| Availability: |
http://jcs.biologists.org/cgi/content/short/116/8/1627; https://doi.org/10.1242/jcs.00336 |
| Rights: |
Copyright (C) 2003, Company of Biologists |
| Accession Number: |
edsbas.E70049FA |
| Database: |
BASE |