| Title: |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
| Authors: |
Jan Winchenbach; Tim Düking; Stefan A. Berghoff; Sina K. Stumpf; Swen Hülsmann; Klaus-Armin Nave; Gesine Saher |
| Source: |
F1000Research, Vol 5 (2016) |
| Publisher Information: |
F1000 Research Ltd |
| Publication Year: |
2016 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Neurogenetics; Medicine; Science |
| Description: |
Background: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under control of the Aldh1l1 regulatory region. Results: When crossbred to Cre reporter mice, adult Aldh1l1-CreERT2 mice show efficient gene targeting in astrocytes. No such Cre-mediated recombination was detectable in CNS neurons, oligodendrocytes, and microglia. As expected, Aldh1l1-CreERT2 expression was evident in several peripheral organs, including liver and kidney. Conclusions: Taken together, Aldh1l1-CreERT2 mice are a useful tool for studying astrocytes in neurovascular coupling, brain metabolism, synaptic plasticity and other aspects of neuron-glia interactions. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://f1000research.com/articles/5-2934/v1; https://doaj.org/toc/2046-1402; https://doaj.org/article/53c8f73488c14ec299224e06b6b62fe4 |
| DOI: |
10.12688/f1000research.10509.1 |
| Availability: |
https://doi.org/10.12688/f1000research.10509.1; https://doaj.org/article/53c8f73488c14ec299224e06b6b62fe4 |
| Accession Number: |
edsbas.E72E1E63 |
| Database: |
BASE |