| Title: |
Identification of Borrelia protein candidates in mouse skin for potential diagnosis of disseminated Lyme borreliosis |
| Authors: |
Grillon, Antoine; Westermann, Benoît; Cantero Mendieta, Liz Paola; Jaulhac, Benoit; Voordouw, Maarten; Kapps, Delphine; Collin, Elody; Barthel, Cathy; Ehret-Sabatier, Laurence; Boulanger, Nathalie |
| Contributors: |
Virulence bactérienne précoce : fonctions cellulaires et contrôle de l'infection aiguë et subaiguë (VBP); Université de Strasbourg (UNISTRA); Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC); Institut Pluridisciplinaire Hubert Curien (IPHC); Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS); Architecture et Réactivité de l'ARN (ARN); Institut de biologie moléculaire et cellulaire (IBMC); Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS); ANR-16-CE17-0003,DIABOLYC,Diagnostic cutané de la borréliose de Lyme tardive(2016) |
| Source: |
ISSN: 2045-2322. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2017 |
| Collection: |
HAL-IN2P3 (Institut national de physique nucléaire et de physique des particules) |
| Subject Terms: |
Flagellin; Tandem Mass Spectrometry; Skin; Real-Time Polymerase Chain Reaction; Peptides; Inbred C3H; Mice; Lyme Disease; Ixodes; Female; Bacterial; DNA; High Pressure Liquid; Chromatography; Borrelia; Bacterial Proteins; Animals; Adrenal Cortex Hormones; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| Description: |
In vector-borne diseases, the skin plays an essential role in the transmission of vector-borne pathogens between the vertebrate host and blood-feeding arthropods and in pathogen persistence. Borrelia burgdorferi sensu lato is a tick-borne bacterium that causes Lyme borreliosis (LB) in humans. This pathogen may establish a long-lasting infection in its natural vertebrate host where it can persist in the skin and some other organs. Using a mouse model, we demonstrate that Borrelia targets the skin regardless of the route of inoculation, and can persist there at low densities that are difficult to detect via qPCR, but that were infective for blood-feeding ticks. Application of immunosuppressive dermocorticoids at 40 days post-infection (PI) significantly enhanced the Borrelia population size in the mouse skin. We used non-targeted (Ge-LC-MS/MS) and targeted (SRM-MS) proteomics to detect several Borrelia-specific proteins in the mouse skin at 40 days PI. Detected Borrelia proteins included flagellin, VlsE and GAPDH. An important problem in LB is the lack of diagnosis methods capable of detecting active infection in humans suffering from disseminated LB. The identification of Borrelia proteins in skin biopsies may provide new approaches for assessing active infection in disseminated manifestations. |
| Document Type: |
article in journal/newspaper |
| Language: |
French |
| DOI: |
10.1038/s41598-017-16749-9 |
| Availability: |
https://hal.science/hal-02628398; https://hal.science/hal-02628398v1/document; https://hal.science/hal-02628398v1/file/Grillon_et_al-2017-Scientific_Reports.pdf; https://doi.org/10.1038/s41598-017-16749-9 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.E74ECB73 |
| Database: |
BASE |