| Title: |
Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People |
| Authors: |
Demaret, Julie; Alidjinou, E. K.; Goffard, Anne; Trauet, Jacques; Miczek, S.; Vuotto, F.; Dendooven, Arnaud; Huvent-Grelle, D.; Podvin, J.; Dreuil, D.; Faure, Karine; Deplanque, Dominique; Bocket, L.; Duhamel, A.; Labreuche, J.; Sobaszek, A.; Hisbergues, M.; Puisieux, F.; Labalette, Myriam; Lefevre, Guillaume |
| Contributors: |
Université de Lille; Inserm; CHU Lille; Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017; Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286; Lille Neurosciences & Cognition (LilNCog) - U 1172; Institute for Translational Research in Inflammation - U 1286 INFINITE |
| Publication Year: |
2022 |
| Collection: |
LillOA (Lille Open Archive - Université de Lille) |
| Subject Terms: |
mRNA vaccination; T cells response; older people and ageing; vaccine; "SARS - CoV - 2" |
| Description: |
Long-term care facility (LTCF) older residents display physiological alterations of cellular and humoral immunity that affect vaccine responses. Preliminary reports suggested a low early postvaccination antibody response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to focus on the specific T-cell response. We quantified S1-specific IgG, neutralizing antibody titers, total specific IFNγ-secreting T cells by ELISpot, and functionality of CD4+- and CD8+-specific T cells by flow cytometry, after two doses of the BNT162b2 vaccine in younger and older people, with and without previous COVID-19 infection (hereafter referred to as COVID-19-recovered and COVID-19-naive subjects, respectively). Frailty, nutritional, and immunosenescence parameters were collected at baseline in COVID-19-naive older people. We analyzed the immune response in 129 young adults (median age 44.0 years) and 105 older residents living in a LCTF (median age 86.5 years), 3 months after the first injection. Humoral and cellular memory responses were dramatically impaired in the COVID-19-naive older (n = 54) compared with the COVID-19-naive younger adults (n = 121). Notably, older participants’ neutralizing antibodies were 10 times lower than the younger’s antibody titers (p < 0.0001) and LCTF residents also had an impaired functional T-cell response: the frequencies of IFNγ+ and IFNγ+IL-2+TNFα+ cells among specific CD4+ T cells, and the frequency of specific CD8+ T cells were lower in COVID-19-naive older participants than in COVID-19-naive young adults (p < 0.0001 and p=0.0018, respectively). However, COVID-19-recovered older participants (n = 51) had greater antibody and T-cell responses, including IFNγ+ and IFNγ+IL-2+TNFα+-specific CD4+ T cells (p < 0.0001), as well as TNFα+-specific CD8+ T cells (p < 0.001), than COVID-19-naive older adults. We also observed that “inflammageing” and particularly high plasma levels of TNFα was associated to poor antibody response in the older ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/octet-stream |
| Language: |
English |
| Relation: |
Frontiers in Immunology; Front. Immunol.; http://hdl.handle.net/20.500.12210/69272 |
| Availability: |
https://hdl.handle.net/20.500.12210/69272 |
| Rights: |
Attribution 3.0 United States ; info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.E767CA6F |
| Database: |
BASE |