| Title: |
The testicular transcriptome associated with spermatogonia differentiation initiated by gonadotrophin stimulation in the juvenile rhesus monkey (Macaca mulatta) |
| Authors: |
Ramaswamy, Suresh; Walker, William H.; Aliberti, Paula; Sethi, Rahil; Marshall, Gary R.; Smith, Alyxzandria; Nourashrafeddin, Seyedmehdi; Belgorosky, Alicia; Chandran, Uma R.; Hedger, Mark P.; Plant, Tony M. |
| Publisher Information: |
Oxford University Press |
| Collection: |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| Subject Terms: |
Differentiation; Glial Cell Line-Derived Neurotrophic Factor Family Receptor Alpha 1; Global Gene Expression; Gonadotrophin; Retinoic Acid; Rhesus Monkey; Rna-Seq; Spermatogenesis- a Nd Oogenesis-Specific Basic Helix-Loop-Helix 1; Spermatogonia; Testis; https://purl.org/becyt/ford/3.2; https://purl.org/becyt/ford/3 |
| Description: |
STUDY QUESTION What is the genetic landscape within the testis of the juvenile rhesus monkey (Macaca mulatta) that underlies the decision of undifferentiated spermatogonia to commit to a pathway of differentiation when puberty is induced prematurely by exogenous LH and FSH stimulation? SUMMARY ANSWER Forty-eight hours of gonadotrophin stimulation of the juvenile monkey testis resulted in the appearance of differentiating B spermatogonia and the emergence of 1362 up-regulated and 225 down-regulated testicular mRNAs encoding a complex network of proteins ranging from enzymes regulating Leydig cell steroidogenesis to membrane receptors, and from juxtacrine and paracrine factors to transcriptional factors governing spermatogonial stem cell fate. WHAT IS KNOWN ALREADY Our understanding of the cell and molecular biology underlying the fate of undifferentiated spermatogonia is based largely on studies of rodents, particularly of mice, but in the case of primates very little is known. The present study represents the first attempt to comprehensively address this question in a highly evolved primate. STUDY DESIGN, SIZE, DURATION Global gene expression in the testis from juvenile rhesus monkeys that had been stimulated with recombinant monkey LH and FSH for 48 h (N = 3) or 96 h (N = 4) was compared to that from vehicle treated animals (N = 3). Testicular cell types and testosterone secretion were also monitored. PARTICIPANTS/MATERIALS, SETTING, METHODS Precocious testicular puberty was initiated in juvenile rhesus monkeys, 14-24 months of age, using a physiologic mode of intermittent stimulation with i.v. recombinant monkey LH and FSH that within 48 h produced â adult' levels of circulating LH, FSH and testosterone. Mitotic activity was monitored by immunohistochemical assays of 5-bromo-2′-deoxyuridine and 5-ethynyl-2′-deoxyuridine incorporation. Animals were bilaterally castrated and RNA was extracted from the right testis. Global gene expression was determined using RNA-Seq. Differentially expressed genes (DEGs) were ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/32/10/2088/4098165; https://hdl.handle.net/11336/72271; CONICET Digital; CONICET |
| Availability: |
https://hdl.handle.net/11336/72271 |
| Rights: |
info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| Accession Number: |
edsbas.E8079488 |
| Database: |
BASE |