| Title: |
A Comparison of Cell Activation, Exhaustion, and Expression of HIV Coreceptors and Restriction Factors in HIV-1-and HIV-2-Infected Nonprogressors ; AIDS Res Hum Retroviruses |
| Authors: |
DIALLO, M. S.; SAMRI, A.; CHARPENTIER, C.; BERTINE, M.; CHEYNIER, R.; THIEBAUT, Rodolphe; MATHERON, S.; COLLIN, F.; BRAIBANT, M.; CANDOTTI, D.; BRUN-VEZINET, F.; AUTRAN, B. |
| Publication Year: |
2020 |
| Subject Terms: |
Sciences du Vivant [q-bio]/Santé publique et épidémiologie |
| Description: |
Human immunodeficiency viruses induce rare attenuated diseases due either to HIV-1 in the exceptional long-term nonprogressors (LTNPs) or to HIV-2 in West Africa. To better understand characteristics of these two disease types we performed a multiplex comparative analysis of cell activation, exhaustion, and expression of coreceptors and restriction factors in CD4 T cells susceptible to harbor those viruses. We analyzed by flow cytometry the expression of HLA-DR, PD1, CCR5, CXCR6, SAMHD1, Blimp-1, and TRIM5 alpha on CD4 T cell subsets from 10 HIV-1(+) LTNPs and 14 HIV-2(+) (12 nonprogressors and 2 progressors) of the ANRS CO-15 and CO-5 cohorts, respectively, and 12 HIV- healthy donors (HD). The V3 loop of the HIV-1 envelope from 6 HIV-1+ LTNPs was sequenced to determine the CXCR6-binding capacity. Proportions of HLA-DR+ and PD1+ cells were higher in memory CD4 T subsets from HIV-1 LTNPs compared with HIV-2 and HD. Similar findings were observed for CCR5+ cells although limited to central-memory CD4 T cell (TCM) and follicular helper T cell subsets, whereas all major subsets from HIV-1 LTNPs contained less CXCR6+ cells compared with HIV-2. All six V3 loop sequences from HIV-1 LTNPs contained a proline at position 326. Proportions of SAMHD1+ cells were higher in all resting CD4 T subsets from HIV-1 LTNPs compared with the other groups, whereas Blimp-1+ and Trim5 alpha+ cells did not differ. The CD4 T cell subsets from HIV-1 LTNPs differ from those of HIV-2-infected subjects by higher levels of activation, exhaustion, and SAMHD1 expression that can reflect the distinct patterns of host/virus relationships. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
33050708 |
| DOI: |
10.1089/aid.2020.0084 |
| Availability: |
https://oskar-bordeaux.fr/handle/20.500.12278/26682; https://hdl.handle.net/20.500.12278/26682; https://doi.org/10.1089/aid.2020.0084 |
| Rights: |
open |
| Accession Number: |
edsbas.E869654F |
| Database: |
BASE |