| Title: |
Poster session 1 |
| Authors: |
Andre, E; De Pauw, A; Porporato, P; Bouzin, C; Draoui, N; Sonveaux, P; Balligand, J-L; Pole of Pharmacology and Therapeutics; Yaniz-Galende, E; Mougenot, N; Formicola, L; Nadaud, S; Dierick, F; Hajjar, RJ; Marazzi, G; Sassoon, D; Hulot, J-S; Hamilton, C; Zamora, VR; Burton, FL; Macquaide, N; Smith, GL; Dusting, G J; Hernandez, D; Sivakumaran, P; Millard, R; Wong, RCB; Pebay, A; Shepherd, RK; Lim, SY; Hellen, N; Owen, T; Jabbour, RJ; Kloc, M; Kodagoda, T; Denning, C; Harding, SE; Poulet, CE; Ramos, S; Terracciano, C; Gorelik, J; Diez Cunado, M; Wei, K; Bushway, P; Ruiz-Lozano, P; Mercola, M; Smits, AM; Moerkamp, AT; Vegh, AMD; Dronkers, E; Lodder, K; Van Herwaarden, T; Goumans, MJ; Lowe, V; Pellet-Many, C; Zachary, I; Eckardt, D; Noack, K; Bosio, A; Du Pre, B; Feyen, DAM; Demkes, EJ; Dierickx, PJ; Doevendans, PA; Vos, MA; Van Veen, AAB; Van Laake, LW; Sanz Ruiz, R; Fernandez Santos, ME; Suarez Sancho, S; Fuentes Arroyo, L; Plasencia Martin, V; Velasco Sevillano, P; Casado Plasencia, A; Climent, AM; Guillem, M; Atienza Fernandez, F; Fernandez-Aviles, F; Moerkamp, A T; Dingenouts, CKE; Kruithof, BPT; Tribulova, N; Knezl, V; Szeiffova Bacova, B; Egan Benova, T; Viczenczova, C; Goncalvesova, E; Slezak, J; Smani, T; Calderon-Sanchez, E; Diaz, I; Ordonez, A; Liskova, Y V; Salikova, S P; Greco, S; Zaccagnini, G; Voellenkle, C; Sadeghi, I; Maimone, B; Castelvecchio, S; Gaetano, C; Menicanti, L; Martelli, F; Guzzolino, E; Hatcher, C; D'aurizio, R; Groth, M; Baugmart, M; Mercatanti, A; Russo, F; Mariani, L; Magliaro, C; Pitto, L; Franco, D; Lozano-Velasco, E; Jodar-Garcia, A; Galiano-Torres, J; Lopez-Navarrete, I; Aranega, A; Wagensteen, R; Quesada, A; Knorr, M; Finger, S; Karbach, S; Kossmann, S; Muenzel, T; Wenzel, P; Pavoine, C; Keck, M; Favier, S; Fuand, A; Atassi, F; Barbier, C; Lompre, AM; Hulot, JS; Bukowska, A; Nikonova, Y; Pluteanu, F; Kockskaemper, J; Chilukoti, RK; Wolke, C; Lendeckel, U; Gardemann, A; Goette, A; Van Linthout, S; Miteva, K; Pappritz, K; Mueller, I; El-Shafeey, M; Ringe, J; Tschoepe, C; Sulzgruber, P; Koller, L; Richter, B; Blum, S; Koprak, M; Huelsmann, M; Pacher, R; Goliasch, G; Wojta, J; Niessner, A; Latet, S C; Van Herck, P L; Claeys, M J; Haine, S E; Lenders, G D; Miljoen, H P; Segers, V F; Vandendriescche, T R; Hoymans, V Y; Vrints, C J; Portnychenko, A; Lapikova-Bryhinska, T; Gurianova, V; Portnichenko, H; Vasylenko, M; Zapara, Y; Portnichenko, V; Cannavo, A; Liccardo, D; Lymperopoulos, A; Santangelo, M; Leosco, D; Koch, WJ; Ferrara, N; Rengo, G; Kamilova, U; Alieva, T; Rasulova, Z; Masharipova, D; Sagach, VF; Dorofeyeva, N A; Drachuk, KO; Santin, Y; Sicard, P; Yucel, Y; Dutaur, M; Vindis, C; Parini, A; Mialet-Perez, J; Octavia, Y; Van Deel, ED; De Boer, M; De Waard, MC; Duncker, DJ; Haller, PM; Nagel, F; Inci, M; Santer, D; Hallstroem, S; Podesser, BK; Ludwig Boltzmann Cluster for Cardiovascular Research - Vienna Working Heart Research Group; Kararigas, G; Kietadisorn, R; Swinnen, M; Duimel, H; Verheyen, F; Chrifi, I; Brandt, MM; Cheng, C; Janssens, S; Moens, AL; Rubies, C; Batlle, M; Dantas, AP; Sanz, M; Sitges, M; Mont, L; Guasch, E; Dei Zotti, F; Lobysheva, I; Beauloye, C; Pole of pharmacology; Wong, KHK; Vanhoutte, PM; Tang, EHC; Gonzalez Miqueo, A; Beaumont, J; Lopez, B; Ravassa, S; Hermida, N; Valencia, F; Gomez-Doblas, JJ; San Jose, G; De Teresa, E; Diez, J; Van De Merbel, AF; Kruithof-De Julio, M; Kadur Nagaraju, C; Claus, P; Dries, E; Angelo Singh, A; Vermeulen, K; Roderick, HL; Sipido, KR; Driesen, RB; Shaposhnikova, Y; Ilchenko, I; Bobronnikova, L; Songia, P; Myasoedova, V; Alamanni, F; Tremoli, E; Poggio, P; Lindner, D; Becher, PM; Gotzhein, F; Klingel, K; Blankenberg, S; Westermann, D; Wilson, C; Zi, M; Cartwright, E; Benzoni, P; Campostrini, G; Bonzanni, M; Milanesi, R; Bucchi, A; Baruscotti, M; Difrancesco, D; Barbuti, A; Fabbri, A; Fantini, M; Wilders, R; Severi, S; Computational Physiopathology Unit; Dell' Era, P; Serzanti, M; Olesen, MS; Muneretto, C; Bisleri, G; Jorge, E; Amoros-Figueras, G; Raga, S; Campos, B; Alonso-Martin, C; Rodriguez-Font, E; Vinolas, X; Cinca, J; Guerra, JM; Casini, S; Mengarelli, I; Schumacher, CA; Veldkamp, MW; Verkerk, AO; Remme, CA; Veerman, C; Guan, K; Stauske, M; Tan, H; Barc, J; Wilde, A; Verkerk, A; Bezzina, C; Nikolov, A; Tsinlikov, I; Tsinlikova, I; Nicoloff, G; Blazhev, A; Garev, A; Bublikov, DS; Andrienko, AV; Lychev, VG; Vorobova, EN; Anchugina, DA; Kheloufi, M; Vion, AC; Hammoutene, A; Poisson, J; Dupont, N; Souyri, M; Tedgui, A; Codogno, P; Boulanger, CM; Rautou, PE; Torres, M; Montserrat, JM; Almendros, I; Walker, R E; Austin, C A; Holt, C M; Van Dijk, RA; Rijs, K; Wezel, A; Hamming, JF; Kolodgie, FD; Virmani, R; Schaapherder, AF; Lindeman, JHN; NA; Posthuma, JJ; Posma, JJN; Van Oerle, R; Spronk, HMH; Ten Cate, H; Laboratory for Clinical Thrombosis and Haemostasis; Dumitriu, I E; Dinkla, S; Kaski, JC; Karshovska, E; Schober, A; Sakic, A; Chaabane, C; Ambartsumian, N; Grigorian, M; Bochaton-Piallat, ML; Alexandru, N; Dragan, E; Andrei, E; Niculescu, L; Georgescu, A; Pathophysiology and Pharmacology Department; Martin-Lorenzo, M; Gonzalez-Calero, L; Maroto, AS; Martinez, PJ; Heredero, A; Aldamiz-Echevarria, G; Vivanco, F; Alvarez-Llamas, G; Molica, F; Meens, MJ; Pelli, G; Foglia, B; Scemes, E; Kwak, BR; Taylor, RF; Caldwell, JL; Eisner, DA; Dibb, KM; Trafford, AW; Mcarthur, L; Chilton, L; Smith, G L; Nicklin, S A; Crocini, C; Coppini, R; Ferrantini, C; Yan, P; Loew, LM; Poggesi, C; Cerbai, E; Pavone, F S; Sacconi, L; Matsuyama, T-A; Tanaka, H; Ishibashi-Ueda, H; Takamatsu, T; Mazzoni, L; Gentile, F; Pioner, JM; Santini, L; Sartiani, L; Bargelli, V; Mugelli, A; Lin, W K; Maciejewska, M; Bolton, E L; Wang, Y; O'brien, F; Ruas, M; Lei, M; Sitsapesan, R; Galione, A; Terrar, D A; Owen, TJ; Smith, JG; Garcia, D; Barriales-Villa, R; Monserrat, L; Marston, SB; Scigliano, M; Watson, S; Tkach, S; Faggian, G; Terracciano, C M; Perbellini, F; Sheehan, A; Eiros Zamora, J; Papadaki, M; Messer, A; Marston, S; Gould, I; Bezerra Gurgel, A R; Johnston, A; Dunne, M; Smith, G; Kemi, OJ; Bromage, DI; Pillai, M; Davidson, SM; Yellon, DM; Kiss, A; Tratsiakovich, Y; Jang, J; Gonon, AT; Pernow, J; W.G. on Cellular Biology of the Heart; Ikeda, G; Matoba, T; Koga, J; Egashira, K; Pickard, J M J; Burke, N; Wirth, G; Korpisalo, P; Hakkarainen, H; Laidinen, S; Yla-Herttuala, S; Casos, K; Ferrer-Curriu, G; Perez, M; Permanyer, E; Blasco-Lucas, A; Gracia, JM; Castro, MA; Barquinero, J; Galinanes, M; Khudiakov, A; Kostina, D; Kostareva, A; Malashicheva, A; Nistal, JF; Merino, D; Ruiz, L; Gomez, J; Juarez, C; Gil, A; Garcia, R; Hurle, MA; Rossi, A; Tesi, C; Belardinelli, L; Olivotto, I; Park, S-J; Eun-Ji, EJ; Lim, B-K; Choi, D-J; Di Maggio, S; Milano, G; Bertolotti, M; De Marchis, F; Zollo, F; Sommariva, E; Capogrossi, M C; Pompilio, G; Bianchi, M E; Raucci, A; Scellini, B; Tardiff, J; Dini, L; Diolaiuti, L; Ferrari, P; Buyandelger, B; Mansfield, C; Luther, P; Knoell, R; Larrasa-Alonso, J; Villalba, M; Sanchez-Cabo, F; Lopez-Olaneta, MM; Ortiz-Sanchez, P; Garcia-Pavia, P; Lara-Pezzi, E; Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III. Myocardial Biology Programme. Group of Molecular Regulation of Heart Failure; Schirmer, I; Klauke, B; Gerdes, D; Schulz, U; Gummert, J; Milting, H; Erich and Hanna Klessmann-Institute for Cardiovascular Research and Development; Chin, SH; Wake, E; Kocsis-Fodor, G; Brack, KE; Ng, GA; Cimiotti, D; Smolina, N; Majchrzak, M; Moehner, D; Wies, A; Stehle, R; Pfitzer, G; Muegge, A; Jaquet, K; Martini, H; Maggiorani, D; Lefevre, L; Cussac, D; Douin-Echinard, V; Hohensinner, P J; Ebenbauer, B; Kaun, C; Prager, M; Rega-Kaun, G; Garabito, M; Costa, G; Onetti, Y; Jimenez-Altayo, F; Vila, E; Dantas, A P; Zeni, F; Scopece, A; Piacentini, L; Bianchi, ME; Capogrossi, MC; Colombo, G; Licholai, S; Blaz, M; Kapelak, B; Sanak, M; De Bortoli, M; Bauce, B; Calore, C; Lorenzon, A; Calore, M; Poloni, G; Mazzotti, E; Rigato, I; Daliento, L; Basso, C; Thiene, G; Melacini, P; Corrado, D; Rampazzo, A; Sivitskaya, L; Danilenko, NG; Vaikhanskaya, TG; Davydenko, OG; Sivitskaya LN; Kura, B; Yin, CH; Kukreja, R; Rainer, PP; Lee, DI; Sorge, M; Glabe, C; Paolocci, N; Guarnieri, C; Tomaselli, GF; Kass, DA; Van Eyk, JE; Agnetti, G; Smith, LE; Cordwell, SJ; White, MY; Suna, G; Wojakowski, W; Lynch, M; Barallobre-Barreiro, J; Yin, X; Mayr, U; White, S; Jahingiri, M; Hill, J; Mayr, M; Gambardella, J; Sorriento, D; Ciccarelli, M; Fiordelisi, A; Campiglia, P; Trimarco, B; Iaccarino, G; Cozma, A; Sitar Taut, AV; Schiau, S; Orasan, O; Halloumi, W; Negrean, V; Zdrenghea, D; Pop, D; De Gonzalo Calvo, D; Van Der Meer, RW; Rijzewijk, LJ; Smit, JWA; Revuelta-Lopez, E; Nasarre, L; Escola-Gil, JC; Lamb, HJ; Llorente-Cortes, V; Scoditti, E; Pellegrino, M; Massaro, M; Carluccio, MA; Calabriso, N; Wabitsch, M; Storelli, C; De Caterina, R; Clark, B J; Church, S J; Callagy, S; Begley, P; Kureishy, N; Mcharg, S; Bishop, P N; Unwin, R D; Cooper, G J S; Mawad, D; Tonkin, J; Bello, S O; Simonotto, J D; Lyon, A R; Stevens, M M; Harding, S E; Kernbach, M; Czichowski, V; Gomez, L; Fuentes, L; Hernandez-Redondo, I; Guillem, MS; Fernandez, ME; Sanz, R; Atienza, F; Llucia-Valldeperas, A; Soler-Botija, C; Prat-Vidal, C; Galvez-Monton, C; Roura, S; Perea-Gil, I; Bragos, R; Bayes-Genis, A |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2016 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Friday 8 July 2016 |
| Description: |
Emerging evidence supports a mandatory metabolic shift from glycolysis to oxidative phosphorylation during stem cells differentiation. In this study, we investigate cardiac progenitor cells (CPC) metabolic adaptations to a hypoxic environment and the bioenergetic transition underlying cardiomyocyte differentiation process. We intend to identify key metabolic regulators which could be modulated to promote survival/proliferation/differentiation of CPC during the early steps of therapeutic interventions. Under normoxia (21%O2), glucose consumption and lactate release were significantly higher in Sca1+ CPC (isolated from adult mouse hearts by MACS separation) than in neonatal rat cardiac myocytes (NRCM) with a ratio of 2 moles of lactate released per mole of glucose supporting a high glycolytic metabolism. Glucose consumption and lactate release were increased in hypoxia (1%O2), together with increased abundance of the monocarboxylic transporter MCT4 (lactate efflux mediator), of Glut-1 and of PFKFB3 (key regulator of glycolytic rate). BrdU incorporation in CPC was critically dependent on pyruvate, glucose and glutamine availability under normoxia and hypoxia. Oxygen consumption analysis indicated that CPC and NRCM display active mitochondrial ATP production. Notably, basal and maximal respiration were higher in NRCM compared to CPC. Consistently, mitochondrial populations were differentially active in both cell types, as reflected by a 40% decrease in tetramethylrhodamine methyl ester staining intensity (flow cytometry) in CPC compared to NRCM. Moreover, the expression of TOM20 (translocase of outer mitochondrial membrane) was also 50% lower in CPC compared to NRCM. This CPC phenotype dramatically changed upon differentiation (azacytidine/TGFβ/ascorbic acid), with a reduction by third of glucose consumption and lactate release compared to proliferative CPC, in parallel with increased expression of sarcomeric proteins (cardiac TnI, alpha-sr-actinin). In conclusion, despite active mitochondrial ATP production, ... |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S16; http://dx.doi.org/10.1093/cvr/cvw135 |
| DOI: |
10.1093/cvr/cvw135 |
| Availability: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S16; https://doi.org/10.1093/cvr/cvw135 |
| Rights: |
Copyright (C) 2016, European Society of Cardiology |
| Accession Number: |
edsbas.E96CF058 |
| Database: |
BASE |