| Title: |
Effect of the deletion of KAR4 on mating gene induction. |
| Authors: |
Sandrine Pinheiro; Mariona Nadal-Ribelles; Carme Solé; Vincent Vincenzetti; Yves Dusserre; Francesc Posas; Serge Pelet |
| Publication Year: |
2025 |
| Collection: |
The University of Auckland: Figshare |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Immunology; Developmental Biology; Science Policy; Infectious Diseases; Plant Biology; Biological Sciences not elsewhere classified; two haploid cells; saccharomyces cerevisiae < p; core promoter modulate; ste12 binding sites; binding sites; strong dimer; specific timing; largely encoded; large diversity; basal association |
| Description: |
A. Dynamics of nuclear enrichment of the p AGA1 -dSPTR-Y in WT (solid lines) and kar4∆ cells (dashed lines). B. Histograms of the difference in response time between the tested promoter and the internal p AGA1 -dPSTR Y reference for WT (solid lines) and kar4∆ cells (dashed lines) for two different non-consensus PRE sequences associated to one consensus PRE. C. Summary graph displaying the expression output, the speed and the fraction of responding cells for promoters with various PRE conformations in WT and kar4∆ cells. The color of the marker indicates the difference in response time between the synthetic promoter and the reference p AGA1 -dPSTR Y . The size of the marker represents the fraction of responding cells. The expression output of individual replicates is indicated by small white dots. The dashed line represents the expression output and the dashed dotted line the expression threshold calculated based on the p SYN 3TT in WT cells. The O and T indicate a significant difference between the mean of the replicates (t-test: p-val < 0.05) in the timing of induction (T) or in the expression output (O) between the WT and kar4∆ strains for the same promoter. D. Dynamics of nuclear enrichment of the p SYN -dPSTR R variants (right panel) and p AGA1 -dSPTR-Y (left panel) in WT (solid lines) and kar4∆ cells (dashed lines). E. Dynamics of nuclear enrichment of the p SYN -dPSTR R with two PRE spaced by 3 bp in tail to tail orientation with one mutated PRE (TcAAAC) in WT (solid lines) and kar4∆ cells (dashed line) with the chimeric Ste12-EV promoter and stimulated with β-estradiol at time 0. F. Expression output of the strains measured in panel C. The O indicates that the pSYN 3TT expresses significantly stronger than the two strain with the mutated PRE, which both express to the same level. (PDF) |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Effect_of_the_deletion_of_KAR4_on_mating_gene_induction_/29332261 |
| DOI: |
10.1371/journal.pgen.1011710.s006 |
| Availability: |
https://doi.org/10.1371/journal.pgen.1011710.s006; https://figshare.com/articles/journal_contribution/Effect_of_the_deletion_of_KAR4_on_mating_gene_induction_/29332261 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.E9F7B162 |
| Database: |
BASE |