| Title: |
Calibration of Flow Cytometers Enables Reproducible Measurements of Extracellular Vesicle Concentrations and Reference Range Establishment |
| Authors: |
Bettin, Britta, A; Li, Bo; Falkena, Kim; van Leeuwen, Ton, G; Gollwitzer, Christian; Varga, Zoltán; Ajzenberg, Nadine; Antovic, Jovan, P; Berckmans, Pascale; Buzas, Edit, I; Carney, Randy, P; Cook, Sean; Dignat‐george, Françoise; Faille, Dorothee; Giebel, Bernd; Jones, Jennifer, C; Kim, Yohan; Lacroix, Romaric; Lannigan, Joanne; Lucien, Fabrice; Maaninka, Katariina; de Menezes, Erika, G Marques; Meyer, Annette; Mizenko, Rachel, R; Nelissen, Inge; Nolan, John; Norris, Philip, J; Pink, Desmond; Poudel, Sumeet; Robert, Stéphane; Siljander, Pia R.‐m.; Tang, Vera, A; Tertel, Tobias; Broeck, Tina, van Den; Wang, Lili; Welsh, Joshua, A; Nieuwland, Rienk; van der Pol, Edwin |
| Contributors: |
Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN); Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Assistance Publique - Hôpitaux de Marseille (APHM) |
| Source: |
ISSN: 2001-3078 ; Journal of Extracellular Vesicles ; https://amu.hal.science/hal-05513495 ; Journal of Extracellular Vesicles, 2025, 14 (12), pp.e70189. ⟨10.1002/jev2.70189⟩. |
| Publisher Information: |
CCSD; Taylor & Francis |
| Publication Year: |
2025 |
| Collection: |
Aix-Marseille Université: HAL |
| Subject Terms: |
reference ranges; reproducibility; standardization Rienk; flow cytometry; extracellular vesicles; calibration; blood plasma; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; The concentration of cells is a key component of modern blood tests. Given the biomarker potential of extracellular vesicles (EVs) in blood, we aimed to establish reference ranges for blood cell-derived EVs using flow cytometry. To address the orders-of-magnitude variability in reported EV concentrations between different flow cytometers (FCMs), we first validated a calibration methodology to enable reproducible EV concentration measurements. The methodology was evaluated in an interlaboratory comparison study and shows that calibration reduces the median absolute deviation of EV concentrations measured on 25 different FCMs from 67 % to 25 %-31 %. The calibration methodology was then used to determine reference ranges of erythrocyte-, leukocyte-, and plateletderived EVs in human blood plasma in a cohort of healthy individuals (n = 224). This study demonstrates that calibration enables comparable concentration measurements of blood cell-derived EVs, thereby bringing EVs one step closer to clinical applications. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41392534; PUBMED: 41392534; WOS: 001638613500001 |
| DOI: |
10.1002/jev2.70189 |
| Availability: |
https://amu.hal.science/hal-05513495; https://amu.hal.science/hal-05513495v1/document; https://amu.hal.science/hal-05513495v1/file/J%20of%20Extracellular%20Vesicle%20-%202025%20-%20Bettin%20-%20Calibration%20of%20Flow%20Cytometers%20Enables%20Reproducible%20Measurements%20of.pdf; https://doi.org/10.1002/jev2.70189 |
| Rights: |
https://creativecommons.org/licenses/by-nc/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.EA7964AD |
| Database: |
BASE |