| Title: |
Dissection of alpha-synuclein specific synaptic functions by gene editing |
| Authors: |
Madhivanan, Kayalvizhi; Parra-Rivas, Leonardo A; Joensuu, Merja; Sanders, Shanley; Caballero-Florán, René; Bingham, Dominic; Sharma, Rohan; Jenkins, Paul M; Leterrier, Christophe; Meunier, Frederic A; Roy, Subhojit |
| Publisher Information: |
Wiley Periodicals, Inc. |
| Publication Year: |
2023 |
| Collection: |
University of Michigan: Deep Blue |
| Subject Terms: |
Neurology and Neurosciences; Health Sciences |
| Description: |
BackgroundDespite almost two decades of research, the physiologic role of α-synuclein remains unclear. Mice lacking α-syn only show mild phenotypes, and most mechanistic studies have been done either in α-syn over-expressing systems, or in mice lacking all three synuclein genes (α/β/γ). However, over-expression of proteins can induce unwanted phenotypes, and ‘triple-knockout’ synuclein mice have structural and physiologic changes that are not specific to α-syn – complicating interpretations.MethodsWe used CRISPR technology to systematically inactivate or activate each synuclein gene in cultured mouse hippocampal neurons, ensuring that our manipulations did not lead to compensatory changes in the non-targeted synucleins. Gene-editing was followed by unbiased evaluation of physiology and ultrastructure using optical synaptic vesicle (SV) recycling assays, electrophysiologic recordings, single-molecule trafficking assays, super-resolution imaging, and electron microscopy.ResultsNeurotransmission is maintained at a range of different activity patterns, motivating us to evaluate the role of α-syn under basal states as well as conditions involving trains of action potentials. Elimination of α-syn led to substantial increases in basal exocytosis. Single-molecule trafficking of SVs in these neurons indicated an increase in mobility of reserve pool vesicles when α-syn was absent. Increasing endogenous α-syn levels by CRISPRa had largely reciprocal effects. Recent studies in our lab found that phosphorylation of α-syn at the Ser129-site is a trigger for α-syn function at the synapse. Augmenting α-syn functionality by constitutive phosphorylation of the α-syn Ser129-site also triggered clustering of distal SV-pools, consistent with a role for α-syn in physiologic clustering of reserve pool vesicles. However, when neuronal activity was augmented by trains of action potentials, loss of α-syn selectively attenuated endocytosis, with no effect on exocytosis. Super-resolution imaging revealed that α-syn was translocated to ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
https://hdl.handle.net/2027.42/191906; Alzheimer’s & Dementia |
| DOI: |
10.1002/alz.082510 |
| Availability: |
https://hdl.handle.net/2027.42/191906; https://doi.org/10.1002/alz.082510 |
| Rights: |
IndexNoFollow |
| Accession Number: |
edsbas.EA970A07 |
| Database: |
BASE |