| Title: |
Protective Actions of Anserine Under Diabetic Conditions |
| Authors: |
Verena Peters; Vittorio Calabrese; Elisabete Forsberg; Nadine Volk; Thomas Fleming; Hans Baelde; Tim Weigand; Christian Thiel; Angela Trovato; Maria Scuto; Sergio Modafferi; Claus Peter Schmitt |
| Source: |
International Journal of Molecular Sciences, Vol 19, Iss 9, p 2751 (2018) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2018 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
diabetes; diabetic nephropathy; anserine; carnosine; Hsp70; proteinuria; vascular permeability; Biology (General); QH301-705.5; Chemistry; QD1-999 |
| Description: |
Background/Aims: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. Methods: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured. Results: Anserine has a higher antioxidant capacity compared to carnosine (p < 0.001). In tubular cells (HK-2) stressed with 25 mM glucose or 20–100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels. In HK-2 cells stressed with glucose, co-incubation with anserine also increased hemeoxygenase (HO-1) protein and reduced total protein carbonylation, but had no effect on cellular sirtuin-1 and thioredoxin protein concentrations. Three intravenous anserine injections every 48 h in 12-week-old db/db mice, improved blood glucose by one fifth, vascular permeability by one third, and halved proteinuria (all p < 0.05). Conclusion: Anserine is a potent antioxidant and activates the intracellular Hsp70/HO-1 defense system under oxidative and glycative stress. Short-term anserine treatment in diabetic mice improves glucose homeostasis and nephropathy. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://www.mdpi.com/1422-0067/19/9/2751; https://doaj.org/toc/1422-0067; https://doaj.org/article/74903dc59c514a69a7c138cb59d87045 |
| DOI: |
10.3390/ijms19092751 |
| Availability: |
https://doi.org/10.3390/ijms19092751; https://doaj.org/article/74903dc59c514a69a7c138cb59d87045 |
| Accession Number: |
edsbas.EAF5D3A8 |
| Database: |
BASE |