| Title: |
Next generation microsampling towards sustainable forensic analysis: Volumetric DBS for cocaine and metabolites |
| Authors: |
Palano S.; Turoňova D.; Protti M.; Kujovská Krčmová L.; Sardella R.; Mladěnka P.; Mandrioli R.; Girotti S.; Mercolini L. |
| Contributors: |
Palano S.; Turoňova D.; Protti M.; Kujovská Krčmová L.; Sardella R.; Mladěnka P.; Mandrioli R.; Girotti S.; Mercolini L. |
| Publication Year: |
2024 |
| Collection: |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| Subject Terms: |
Cocaine; Quantitative dried blood spot (qDBS); UHPLC-MS; Whole blood microsampling |
| Description: |
This study introduces for the first time a reliable whole blood microsampling method for forensic analysis of cocaine and its metabolites using quantitative dried blood spot (qDBS) technology and UHPLC-MS analysis. This methodology offers accurate and less invasive sampling and aligns with the current trend towards sustainable and accessible analytical methods. Microsampling is subject-friendly, improves logistics, stability and efficiency, marking a shift towards modern forensic practices with wide-ranging application potential. The qDBS-UHPLC-MS method underwent comprehensive validation, confirming its linearity, sensitivity, precision, extraction efficiency and stability. UHPLC-MS achieved effective chromatographic separation and suitable sensitivity, with detection limits between 1.0 and 2.5 ng/mL, and quantitation limits from 2.5 to 7.5 ng/mL. Analytes showed over 85.1 % extraction yield and less than 7.5 % relative standard deviation in precision. Stability tests indicated superior analyte preservation in qDBS at room temperature versus refrigerated plasma, while minimal matrix effect highlighted the sample clean-up efficiency. Application to real samples yielded consistent quali-quantitative results between qDBS and plasma samples, confirming method suitability for forensic cocaine bioanalysis, thus addressing critical needs in drug testing and pharmaco-toxicological analysis. |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:001332692500001; volume:203; firstpage:1; lastpage:7; numberofpages:7; journal:MICROCHEMICAL JOURNAL; https://hdl.handle.net/11585/972015; https://www.sciencedirect.com/science/article/pii/S0026265X2401049X |
| DOI: |
10.1016/j.microc.2024.110937 |
| Availability: |
https://hdl.handle.net/11585/972015; https://doi.org/10.1016/j.microc.2024.110937; https://www.sciencedirect.com/science/article/pii/S0026265X2401049X |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.EB502C31 |
| Database: |
BASE |