| Title: |
Two-layered susceptibility vessel sign is associated with biochemically quantified thrombus red blood cell content |
| Authors: |
Di Meglio, Lucas D.; Derraz, Imad; Solonomenjanahary, M.; Daly, D.; Chodraui Filho, Salomão Faroj; Ben Maacha, Malek; Labreuche, Julien; Desal, Hubert Armand; Consoli, Arturo; Lapergue, Bertrand; Blanc, Raphaël; Piotin, Michel; Mazighi, Mikael; Ho-Tin-Noé, Benoît; Desilles, Jean Philippe; Bourcier, Romain |
| Contributors: |
Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)); Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord; CHU Rothschild AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU); Neuroradiologie Hôpital Gui de Chauliac; Hôpital Gui de Chauliac CHU Montpellier; Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier); Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS); Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille); Hôpital Guillaume-et-René-Laennec Saint-Herblain; Université de Versailles Saint-Quentin-en-Yvelines (UVSQ); AP‐RM‐17‐005 Institut National de la Santé et de la Recherche Médicale, Inserm; Mary Osborne‐Pellegrin is thanked for help in editing the final draft of the article. This work was supported by INSERM and by La Fondation de l’Avenir (grant number AP‐RM‐17‐005). Dr Di Meglio is the recipient of a PhD grant from La Fondation de L’Avenir.; ANR-18-RHUS-0001,BOOSTER,Brain clOt persOnalized therapeutic Strategies for sTroke Emergent Reperfusion(2018) |
| Source: |
ISSN: 1351-5101. |
| Publisher Information: |
CCSD; Wiley |
| Publication Year: |
2020 |
| Subject Terms: |
predictors; endovascular treatment; acute stroke; [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
| Description: |
International audience ; Background and purpose: Better characterization of the thrombus could be useful to determine acute ischaemic stroke (AIS) aetiology and predict response to thrombolysis and endovascular therapy (EVT). To test the hypothesis that susceptibility vessel sign (SVS) on baseline magnetic resonance imaging (MRI) is related to red blood cell (RBC) content of AIS thrombi, the total haemoglobin contents (HbCs) of AIS thrombi retrieved by EVT from patients with or without SVS or two-layered SVS (TLSVS) were compared. Methods: Baseline MRI of 84 anterior AIS patients was reviewed by neuro-radiologists blinded to clinical and biochemical data. Thrombi from these patients were retrieved by EVT and analysed for HbC by quantitative enzyme-linked immunosorbent assay and measurement of haem concentration. Results: Susceptibility vessel sign and TLSVS were respectively observed in 85.7% and 50.0% of cases. The median HbC content was 253 µg/mg thrombus (interquartile range 177–333) and the median haem content was 219 µg/mg thrombus (131–264). Thrombus HbC and haem content were highly correlated with thrombus RBC content determined by flow cytometry (r = 0.94). Thrombi from patients with TLSVS weighed more [31.1 (16.5–68.3) mg vs. 17.7 (11.7–33.3) mg; P = 0.005] and had a higher HbC content [278 (221–331) µg/mg vs. 196 (139–301) µg/mg; P = 0.010] compared to thrombi from patients without TLSVS. There was no difference in thrombus weight or HbC content according to SVS status. Conclusions: Our study shows that TLSVS is significantly associated with a higher thrombus weight and RBC content, as determined by quantitative assays. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/32243692; PUBMED: 32243692 |
| DOI: |
10.1111/ene.14241 |
| Availability: |
https://hal.science/hal-02927416; https://doi.org/10.1111/ene.14241 |
| Accession Number: |
edsbas.ECC0BB8 |
| Database: |
BASE |