| Title: |
Vesicle adhesion in the electrostatic strong-coupling regime studied by time-resolved small-angle X-ray scattering |
| Authors: |
Komorowski, Karlo; Sztucki, Michael; Sharpnack, Lewis; Brehm, Gerrit; Köster, Sarah; Salditt, Tim; Schaeper, Jannis |
| Contributors: |
Komorowski, Karlo; Sztucki, Michael; Sharpnack, Lewis; Brehm, Gerrit; Köster, Sarah; Salditt, Tim; Schaeper, Jannis |
| Publication Year: |
2020 |
| Collection: |
Georg-August-Universität Göttingen: GoeScholar |
| Subject Terms: |
x-ray scattering; membrane biophysics; microfluidics |
| Description: |
We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in the electrostatic strong-coupling regime induced by divalent ions. The bilayer structure and the interbilayer distance dw between adhered vesicles was studied for different DOPC:DOPS mixtures varying the surface charge density of the membrane, as well as for different divalent ions, such as Ca2+, Sr2+, and Zn2+. The results are in good agreement with the strong coupling theory predicting the adhesion state and the corresponding like-charge attraction based on ion-correlations. Using SAXS combined with the stopped-flow rapid mixing technique, we find that in highly charged bilayers the adhesion state is only of transient nature, and that the adhering vesicles subsequently transform to a phase of multilamellar vesicles, again with an inter-bilayer distance according to the theory of strong binding. Aside from the stopped-flow SAXS instrumentations used primarily for these results, we also evaluate microfluidic sample environments for vesicle SAXS in view of future extension of this work. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
EXC 2067: Multiscale Bioimaging; SFB 1286: Quantitative Synaptologie; SFB 1286 %7C A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren; SFB 1286 %7C B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters |
| DOI: |
10.1039/D0SM00259C |
| Availability: |
https://resolver.sub.uni-goettingen.de/purl?gro-2/64290; https://doi.org/10.1039/D0SM00259C; https://mbexc.uni-goettingen.de/literature/publications/186; https://sfb1286.uni-goettingen.de/literature/publications/72 |
| Rights: |
info:eu-repo/semantics/openAccess ; CC BY-NC 3.0 |
| Accession Number: |
edsbas.ECEFF880 |
| Database: |
BASE |