| Title: |
Alterations in mucosa branched N-glycans lead to dysbiosis and downregulation of ILC3: a key driver of intestinal inflammation |
| Authors: |
Rodrigues, CS; Gaifem, J; Pereira, MS; Alves, MF; Silva, M; Padrão, N; Cavadas, B; Moreira-Barbosa, C; Alves, I; Marcos-Pinto, R; Torres, J; Lavelle, A; Colombel, JF; Sokol, H; Pinho, SS |
| Contributors: |
Instituto de Investigação e Inovação em Saúde |
| Publisher Information: |
Taylor & Francis |
| Publication Year: |
2025 |
| Collection: |
Repositório Aberto da Universidade do Porto |
| Subject Terms: |
ILC3; N-glycans; Intestinal inflammation; Microbiome; Prophylaxis |
| Description: |
Co-funded by the European Union under the Grant Agreement no. [101093997]. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or European Health and Digital Executive Agency (HADEA). Neither the European Union nor the granting authority can be held responsible for them. Salomé S. Pinho and Harry Sokol acknowledge funding from European Crohn’s and Colitis Organisation (ECCO) Pioneer Award 2021; Salomé S. Pinho also acknowledges the International Organization for the study of Inflammatory Bowel Disease (IOIBD).Cláudia Rodrigues thanks the Portuguese Foundation for Science and Technology (FCT) for funding (2020.08422.BD). Joana Gaifem acknowledges funding from European Society of Clinical Microbiology and Infectious Diseases (ESCMID Research Grant, 2022), European Crohn’s and Colitis Organisation (ECCO Grant, 2023) and FCT (DOI 10.54499/ 2020.00088.CEECIND/CP1608/CT0001). Márcia S. Pereira, Mariana Silva, Bruno Cavadas and Inês Alves acknowledge funding from FCT (SFRH/BD/110148/2015, SFRH/BD/136388/2018, CEECINST/00123/2021/CP1772/CT0001 and 2022.00337. CEECIND, respectively). ; The perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation and Inflammatory Bowel Disease (IBD) development. The host mucosa glycans (glycocalyx) creates a major biological interface between gut microorganisms and host immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease onset. However, how changes in mucosa glycosylation shape microbiome and how this impact gut immune response and inflammation remains to be clarified. Here, we revealed that alterations in the expression of complex branched N-glycans at gut mucosa surface, modeled in glycoengineered mice, resulted in dysbiosis, with a deficiency in Firmicutes bacteria. Concomitantly, this mucosa N-glycan switch was associated with a downregulation of type 3 innate lymphoid cells ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| Language: |
English |
| Relation: |
info:eu-repo/grantAgreement/EC/HE/101093997/EU; info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.08422.BD/PT; info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F110148%2F2015/PT; info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F136388%2F2018/PT; info:eu-repo/grantAgreement/FCT/CEEC INST 2ed/CEECINST%2F00123%2F2021%2FCP1772%2FCT0001/PT; Gut microbes, vol. 17(1):2461210; https://hdl.handle.net/10216/165231 |
| DOI: |
10.1080/19490976.2025.2461210 |
| Availability: |
https://hdl.handle.net/10216/165231; https://doi.org/10.1080/19490976.2025.2461210 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.EDE226 |
| Database: |
BASE |