| Title: |
The molecular logic of Gtr1/2- and Pib2-dependent TORC1 regulation in budding yeast |
| Authors: |
Cecil, Jacob H; Padilla, Cristina M; Lipinski, Austin A; Langlais, Paul; Luo, Xiangxia; Capaldi, Andrew P |
| Contributors: |
National Institute of General Medical Sciences |
| Source: |
eLife ; volume 13 ; ISSN 2050-084X |
| Publisher Information: |
eLife Sciences Publications, Ltd |
| Publication Year: |
2025 |
| Collection: |
eLife (E-Journal - via CrossRef) |
| Description: |
The Target of Rapamycin kinase Complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. Previous studies have shown that, in Saccharomyces cerevisiae , nitrogen and amino acid signals activate TORC1 via the highly conserved small GTPases, Gtr1/2, and the phosphatidylinositol 3-phosphate binding protein, Pib2. However, it was unclear if/how Gtr1/2 and Pib2 cooperate to control TORC1. Here, we report that this dual regulator system pushes TORC1 into at least three distinct signaling states: (i) a Gtr1/2 on, Pib2 on, rapid growth state in nutrient replete conditions; (ii) a Gtr1/2 inhibited, Pib2 on, adaptive/slow growth state in poor-quality growth medium; and (iii) a Gtr1/2 off, Pib2 off, quiescent state in starvation conditions. We suggest that other signaling pathways work in a similar way to drive a multilevel response via a single kinase, but the behavior has been overlooked since most studies follow signaling to a single reporter protein. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.7554/elife.94628.2 |
| Availability: |
https://doi.org/10.7554/elife.94628.2; https://cdn.elifesciences.org/articles/94628/elife-94628-v1.pdf; https://cdn.elifesciences.org/articles/94628/elife-94628-v1.xml; https://elifesciences.org/articles/94628 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.EE02C868 |
| Database: |
BASE |