| Title: |
Nitric oxide-dependent vasodilation and the regulation of arterial blood pressure |
| Authors: |
FERRARI, ALBERTO; MANCIA, GIUSEPPE; Radaelli, A; Mori, I; Mircoli, L; Perlini, S; Meregalli, P; Fedele, L |
| Contributors: |
Ferrari, A; Radaelli, A; Mori, I; Mircoli, L; Perlini, S; Meregalli, P; Fedele, L; Mancia, G |
| Publisher Information: |
LIPPINCOTT WILLIAMS & WILKINS; PHILADELPHIA |
| Publication Year: |
2001 |
| Collection: |
Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) |
| Subject Terms: |
nitric oxide; L-nitromonomethyl-L-arginine; sympathectomy; ganglionic blockade; spontaneously hypertensive rat |
| Description: |
Conflicting evidence has been reported on the hypothesis that vascular nitric oxide (NO) release is modulated by autonomic influences. Another controversial question is whether an insufficient degree of NO-dependent vasodilation may play a contributory role in the genesis of arterial hypertension. To address these questions we evaluated NO-dependent vasodilation in conscious rats subjected to various experimental manipulations that interfere with autonomic function: chronic chemical sympathectomy (CCSx), acute ganglionic blockade (AGx) and chronic sinoaortic denervation (CSAD). Experiments were also carried out on 6- and 12-week-old spontaneously hypertensive rats (SHR) (i.e. during the pre-hypertensive and the early established hypertensive stage) and in age-matched Wistar-Kyoto (WKY) rats. Nitric oxide-dependent vasodilation was quantified from the extent of blood pressure (BP) elevation in response to acute inhibition of NO synthesis by L-nitromonomethyl-L-arginine (L-NMMA). Chronic chemical sympathectomy was produced by repeated 6-hydroxydopamine injections; AGx was induced by hexamethonium infusion; and CSAD was obtained by aortic nerve section and carotid sinus wall stripping. Nitric oxide synthesis inhibition by L-NMMA was followed by a marked BP elevation in all groups. Rats with CCSx, Agx or CSAD never showed reduced BP responses to L-NMMA compared to intact, control rats. Neither 6- nor 12-week-old SHR had attenuated pressor responses to L-NMMA compared to age-matched WKY rats. In conclusion, the data indicate that (i) in unanaesthetized quietly-behaving rats there is no significant modulation of NO release by autonomic influences and (ii) young SHR have unimpaired NO-dependent vasodilation so it is unlikely that a deficit of vascular NO release plays any etiologic role in the BP elevation of this experimental model. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:000173041600006; volume:38; issue:Suppl. 2; firstpage:S19; lastpage:S22; journal:JOURNAL OF CARDIOVASCULAR PHARMACOLOGY; https://hdl.handle.net/10281/5032 |
| Availability: |
https://hdl.handle.net/10281/5032 |
| Accession Number: |
edsbas.EE19CF50 |
| Database: |
BASE |