| Title: |
Overview of protein misfolding amplification methods and their use in prion disease diagnosis and in investigations of within- and inter-species prion transmission |
| Authors: |
Merve Begum Bacinoglu; Fabio Moda |
| Contributors: |
M.B. Bacinoglu; F. Moda |
| Publication Year: |
2026 |
| Collection: |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| Subject Terms: |
Settore BIOS-09/A - Biochimica clinica e biologia molecolare clinica; Settore BIOS-07/A - Biochimica; Settore MEDS-12/A - Neurologia |
| Description: |
The presentation provides an overview of in vitro protein misfolding amplification assays used in prion research, with particular emphasis on Protein Misfolding Cyclic Amplification (PMCA). After summarizing the principles of prion replication and the limitations of conventional CWD diagnostics, the talk illustrates how RT‐QuIC and PMCA reproduce misfolded prion‐driven conversion mechanisms with high analytical sensitivity. The presentation details the optimization of PMCA conditions using reindeer PRNP variants as substrates and CWD isolates as seeds, highlighting genotype‐dependent differences in amplification efficiency. These experimental insights demonstrate how PMCA can be used not only for sensitive prion detection but also to investigate strain properties, host–substrate compatibility, and the potential impact of PRNP genetic variation on CWD susceptibility. |
| Document Type: |
conference object |
| Language: |
English |
| Relation: |
Project meeting - Chronic wasting disease threatens Fennoscandian reindeer - How will breeding for CWD-resilience affect genetic variation and fitness?; https://hdl.handle.net/2434/1227878 |
| Availability: |
https://hdl.handle.net/2434/1227878 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by-sa/4.0/ |
| Accession Number: |
edsbas.EE5CDEB8 |
| Database: |
BASE |