Molecular structure from models to mastery - a study of human insulin-degrading enzyme in the undergraduate biochemistry laboratory
| Title: | Molecular structure from models to mastery - a study of human insulin-degrading enzyme in the undergraduate biochemistry laboratory |
|---|---|
| Authors: | Araujo, Michael; Barrere, Alexandra A.; Tirado, Selena-Rae; Williams, Candace E.; Strada, Monica I.; Alper, Benjamin J. |
| Publisher Information: | Center for Open Science |
| Publication Year: | 2021 |
| Description: | Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE) to explore structural requirements for enzyme function. Students demonstrated mastery of critical concepts in enzymology including principles of experimental design, implications of enzyme structure-function relationships for molecular evolution, experimental methods for analysis of macromolecular interactions, and computational approaches to scientific inquiry. This investigation was conducted as part of the second-semester undergraduate biochemistry laboratory at Sacred Heart University |
| Document Type: | other/unknown material |
| Language: | unknown |
| DOI: | 10.35542/osf.io/p9egk |
| Availability: | https://doi.org/10.35542/osf.io/p9egk |
| Rights: | http://opensource.org/licenses/MIT |
| Accession Number: | edsbas.EE983D04 |
| Database: | BASE |