| Title: |
Sublethal Supraphysiological Shear Stress Alters Erythrocyte Dynamics in Subsequent Low-Shear Flows |
| Authors: |
McNamee, AP; Fitzpatrick, T; Tansley, GD; Simmonds, MJ |
| Publisher Information: |
Elsevier |
| Publication Year: |
2020 |
| Collection: |
Griffith University: Griffith Research Online |
| Subject Terms: |
Physical sciences; Chemical sciences; Biological sciences |
| Description: |
Blood is a non-Newtonian, shear-thinning fluid owing to the physical properties and behaviors of red blood cells (RBCs). Under increased shear flow, pre-existing clusters of cells disaggregate, orientate with flow, and deform. These essential processes enhance fluidity of blood, although accumulating evidence suggests that sublethal blood trauma—induced by supraphysiological shear exposure—paradoxically increases the deformability of RBCs when examined under low-shear conditions, despite obvious decrement of cellular deformation at moderate-to-higher shear stresses. Some propose that rather than actual enhancement of cell mechanics, these observations are “pseudoimprovements” and possibly reflect altered flow and/or cell orientation, leading to methodological artifacts, although direct evidence is lacking. This study thus sought to explore RBC mechanical responses in shear flow using purpose-built laser diffractometry in tandem with direct optical visualization to address this problem. Freshly collected RBCs were exposed to a mechanical stimulus known to drastically alter cell deformability (i.e., prior shear exposure (PSE) to 100 Pa × 300 s). Samples were subsequently transferred to a custom-built slit-flow chamber that combined laser diffractometry with direct cell visualization. Cell suspensions were sheared in a stepwise manner (between 0.3 and 5.0 Pa), with each step being maintained for 15 s. Deformability and cell orientation indices were recorded for small-scatter Fraunhofer diffraction patterns and also visualized RBCs. PSE RBCs had significantly decreased visualized and laser-derived deformability at any given shear stress ≥1 Pa. Novel, to our knowledge, observations demonstrated that PSE RBCs had increased heterogeneity of direct visualized orientation with flow vector at any shear, which may be due to greater vorticity and thus instability in 5-Pa flow compared with unsheared control. These findings indicate that shear exposure and stress-strain history can alter subsequent RBC behavior in ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
Biophysical Journal; McNamee, AP; Fitzpatrick, T; Tansley, GD; Simmonds, MJ, Sublethal Supraphysiological Shear Stress Alters Erythrocyte Dynamics in Subsequent Low-Shear Flows, Biophysical Journal, 2020; https://hdl.handle.net/10072/399548 |
| DOI: |
10.1016/j.bpj.2020.10.022 |
| Availability: |
https://hdl.handle.net/10072/399548; https://doi.org/10.1016/j.bpj.2020.10.022 |
| Rights: |
© 2020 Biophysical Society. Published by Elsevier Inc. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. ; open access |
| Accession Number: |
edsbas.EED63BA4 |
| Database: |
BASE |