| Title: |
Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage ( Brassica rapa ) |
| Authors: |
Ye-Rin Lee; Chae-Yeon Lim; Sohee Lim; Se Ra Park; Jong-Pil Hong; Jinhee Kim; Hye-Eun Lee; Kisung Ko; Do-Sun Kim |
| Source: |
Plants, Vol 9, Iss 1466, p 1466 (2020) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2020 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Agrobacterium; colorectal carcinoma; EpCAM; plant molecular biopharming; recombinant vaccine; transgenic chinese cabbage; Botany; QK1-989 |
| Description: |
The epithelial cell adhesion molecule (EpCAM) is a tumor-associated antigen and a potential target for tumor vaccine. The EpCAM is a cell-surface glycoprotein highly expressed in colorectal carcinomas. The objective of the present study is to develop an edible vaccine system through Agrobacterium -mediated transformation in Chinese cabbage ( Brassica rapa ). For the transformation, two plant expression vectors containing genes encoding for the EpCAM recombinant protein along with the fragment crystallizable (Fc) region of immunoglobulin M (IgM) and Joining (J)-chain tagged with the KDEL endoplasmic reticulum retention motif (J-chain K) were constructed. The vectors were successfully transformed and expressed in the Chinese cabbage individually using Agrobacterium . The transgenic Chinese cabbages were screened using genomic polymerase chain reaction (PCR) in T 0 transgenic plant lines generated from both transformants. Similarly, the immunoblot analysis revealed the expression of recombinant proteins in the transformants. Further, the T 1 transgenic plants were generated by selfing the transgenic plants (T 0 ) carrying EpCAM–IgM Fc and J-chain K proteins, respectively. Subsequently, the T 1 plants generated from EpCAM–IgM Fc and J-chain K transformants were crossed to generate F 1 plants carrying both transgenes. The presence of both transgenes was validated using PCR in the F 1 plants. In addition, the expression of Chinese cabbage-derived EpCAM–IgM Fc × J-chain K was evaluated using immunoblot and ELISA analyses in the F 1 plants. The outcomes of the present study can be utilized for the development of a potential anti-cancer vaccine candidate using Chinese cabbage. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/2223-7747/9/11/1466; https://doaj.org/toc/2223-7747; https://doaj.org/article/e4989fd3b11f442ca748ff47f377ec50 |
| DOI: |
10.3390/plants9111466 |
| Availability: |
https://doi.org/10.3390/plants9111466; https://doaj.org/article/e4989fd3b11f442ca748ff47f377ec50 |
| Accession Number: |
edsbas.EEDCB86C |
| Database: |
BASE |