| Title: |
Sleep Homeostasis and General Anesthesia ; Are Fruit Flies Well Rested after Emergence from Propofol? |
| Authors: |
Gardner, Benjamin; Strus, Ewa; Meng, Qing Cheng; Coradetti, Thomas; Naidoo, Nirinjini N.; Kelz, Max B.; Williams, Julie A. |
| Source: |
Anesthesiology ; volume 124, issue 2, page 404-416 ; ISSN 0003-3022 |
| Publisher Information: |
Ovid Technologies (Wolters Kluwer Health) |
| Publication Year: |
2016 |
| Description: |
Abstract Propofol produced anesthesia in the fruit fly Drosophila , but it did not dissipate sleep debt or satisfy the homeostatic need for sleep in contrast to rodents. Further studies will be required to validate the findings in both rodents and flies and reconcile the apparent species-specific differences in the interactions between natural sleep and general anesthesia. Background Shared neurophysiologic features between sleep and anesthetic-induced hypnosis indicate a potential overlap in neuronal circuitry underlying both states. Previous studies in rodents indicate that preexisting sleep debt discharges under propofol anesthesia. The authors explored the hypothesis that propofol anesthesia also dispels sleep pressure in the fruit fly. To the authors’ knowledge, this constitutes the first time propofol has been tested in the genetically tractable model, Drosophila melanogaster . Methods Daily sleep was measured in Drosophila by using a standard locomotor activity assay. Propofol was administered by transferring flies onto food containing various doses of propofol or equivalent concentrations of vehicle. High-performance liquid chromatography was used to measure the tissue concentrations of ingested propofol. To determine whether propofol anesthesia substitutes for natural sleep, the flies were subjected to 10-h sleep deprivation (SD), followed by 6-h propofol exposure, and monitored for subsequent sleep. Results Oral propofol treatment causes anesthesia in flies as indicated by a dose-dependent reduction in locomotor activity (n = 11 to 41 flies from each group) and increased arousal threshold (n = 79 to 137). Recovery sleep in flies fed propofol after SD was delayed until after flies had emerged from anesthesia (n = 30 to 48). SD was also associated with a significant increase in mortality in propofol-fed flies (n = 44 to 46). Conclusions Together, these data indicate that fruit flies are effectively anesthetized by ingestion of propofol and suggest that homologous molecular and neuronal targets of ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1097/aln.0000000000000939 |
| Availability: |
https://doi.org/10.1097/aln.0000000000000939; http://pubs.asahq.org/anesthesiology/article-pdf/124/2/404/269679/20160200_0-00027.pdf; http://anesthesiology.pubs.asahq.org/article.aspx?volume=124%26page=404 |
| Accession Number: |
edsbas.EEFA9BF6 |
| Database: |
BASE |