| Title: |
Elevated expression of CYP1A1 and {gamma}-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium |
| Authors: |
Singh, Maneesh N.; Stringfellow, Helen F.; Taylor, Siân E.; Ashton, Kate M.; Ahmad, Mushfika; Abdo, Khalil R.; El-Agnaf, Omar M.A.; Martin-Hirsch, Pierre L.; Martin, Francis L. |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2008 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Articles |
| Description: |
Endometriosis is a debilitating disease in which apoptotic, genetic, immunological, angiogenic and environmental factors have been implicated. Endocrine-disrupting agents (e.g. dioxins) might be involved. Dioxins, via the arylhydrocarbon receptor (AhR), induce estrogen-metabolizing enzymes CYP1A1 and CYP1B1 . Elevated expression of γ-SYNUCLEIN ( γ-SYN ) has been associated with hormone-related conditions. Tissue sets consisting of eutopic and ectopic (ovarian) endometrium from patients with stage 3 or 4 endometriosis were obtained. Following RNA extraction and reverse transcription, quantitative real-time reverse transcriptase–polymerase chain reaction was performed for anti-apoptotic B-cell leukaemia/lymphoma 2 ( BCL-2 ), C YP1A1 , CYP1B1 , estrogen receptor (ER)α, ERβ and γ-SYN . Immunohistochemical analyses for γ-syn, ERα, ERβ and CYP1A1 were also conducted. A 3–9-fold increase in intra-individual expression of CYP1A1 in ectopic (ovarian) endometrium compared with eutopic tissue was observed; immunohistochemical analyses pointed to CYP1A1 being localized to the glandular epithelium. This intra-individual expression profile was not observed for CYP1B1 or BCL-2 . However, a 5–53-fold intra-individual increase in γ-SYN expression was also demonstrated in six of nine tissue sets (a further two showed an increase that was not considered significant) when comparing ectopic to eutopic endometrium; γ-syn positivity was associated with endothelial cells. An elevation in ERβ was also noted when comparing ectopic to eutopic endometrium; with regard to ERα, this was inconsistent. These results suggest an up-regulation of dioxin-inducible CYP1A1 and γ-SYN occurs in endometriosis. Whether γ-syn may be a novel diagnostic marker for endometriosis remains to be ascertained. |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://molehr.oxfordjournals.org/cgi/content/short/14/11/655; http://dx.doi.org/10.1093/molehr/gan056 |
| DOI: |
10.1093/molehr/gan056 |
| Availability: |
http://molehr.oxfordjournals.org/cgi/content/short/14/11/655; https://doi.org/10.1093/molehr/gan056 |
| Rights: |
Copyright (C) 2008, Oxford University Press |
| Accession Number: |
edsbas.EFFE7C7 |
| Database: |
BASE |