| Title: |
Immunization with Recombinant SFTSV/NSs Protein Does Not Promote Virus Clearance in SFTSV-Infected C57BL/6J Mice |
| Authors: |
Liu, Rong; Huang, Dou-Dou; Bai, Jie-Ying; Zhuang, Lu; Lu, Qing-Bin; Zhang, Xiao-Ai; Liu, Wei; Wang, Jing-Yu; Cao, Wu-Chun |
| Contributors: |
Wang, JY (reprint author), Peking Univ, Sch Publ Hlth, Beijing 100191, Peoples R China.; Peking Univ, Sch Publ Hlth, Beijing 100191, Peoples R China.; Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China.; Anhui Med Univ, Grad Sch, Hefei, Peoples R China.; Acad Mil Med Sci, Lab Anim Ctr, Beijing, Peoples R China. |
| Source: |
SCI ; PubMed |
| Publisher Information: |
病毒免疫学 |
| Publication Year: |
2015 |
| Collection: |
Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| Subject Terms: |
THROMBOCYTOPENIA SYNDROME VIRUS; INNATE IMMUNE-RESPONSE; SEVERE FEVER; HEMORRHAGIC-FEVER; CYTOMEGALOVIRUS-INFECTION; NONSTRUCTURAL PROTEINS; NS1; PATHOGENESIS; CHINA; ENCEPHALITIS |
| Description: |
The severe fever with thrombocytopenia syndrome (SFTS), caused by a novel Phlebovirus in the Bunyaviridae family named SFTS virus (SFTSV), is an emerging hemorrhagic fever with a wide distribution and high case-fatality rate. Neither effective treatment nor vaccines are available to treat and prevent this disease to date. It was recently reported that SFTSV nonstructural protein in S segment (SFTSV/NSs) functioned as the interferon (IFN) antagonist targeting for suppressing host's innate immunity. This study was designed to investigate the potential of recombinant SFTSV (rSFTSV)/NSs protein for inducing anti-NSs antibodies by pre-exposure vaccination to block SFTSV/NSs in the SFTSV-infected C57BL/6J mice. All mice in the rSFTSV/NSs-vaccinated group, negative control group, and blank control group survived with no visible clinical abnormities throughout the experiment, except for their sacrifice for sampling at each observation point. However, unexpectedly, a negative effect on the bodyweight of rSFTSV/NSs-vaccinated mice was observed after 21 days postinoculation. Pre-exposure vaccination with rSFTSV/NSs did not accelerate virus removal in mice though high titer of anti-NSs antibodies and elevated IFN-gamma were detected in sera. Before virus challenge, the rSFTSV/NSs-vaccinated mice and negative control mice had a larger amount of platelets (PLT) than the blank control mice, which indicated that Freund's adjuvants could stimulate PLT production. In the aspect of cytokines, the rSFTSV/NSs-vaccinated mice had a 5- to 10-fold increase in interleukin (IL)-2, IL-5, IL-6, IFN-gamma, and tumor necrosis factor-alpha, which probably just had a negative effect on the bodyweight of mice. In general, therefore, previous vaccination with rSFTSV/NSs did not accelerate virus clearance in the SFTSV-infected mice. ... |
| Document Type: |
journal/newspaper |
| Language: |
English |
| ISBN: |
978-0-00-349472-3; 0-00-349472-1 |
| Relation: |
651062; http://hdl.handle.net/20.500.11897/341674; WOS:000349472100007 |
| DOI: |
10.1089/vim.2014.0100 |
| Availability: |
https://hdl.handle.net/20.500.11897/341674; https://doi.org/10.1089/vim.2014.0100 |
| Accession Number: |
edsbas.F0E99F9A |
| Database: |
BASE |