Protocol for modeling the repair of intestinal damage by co-culturing mesenchymal stromal/stem cells and intestinal organoids
| Title: | Protocol for modeling the repair of intestinal damage by co-culturing mesenchymal stromal/stem cells and intestinal organoids |
|---|---|
| Authors: | Skarp,Kari Pekka; Yetkin-Arik, Bahar; Jansen, Suze A.; Lindemans, Caroline A.; Lorenowicz, Magdalena J.; Child Health; Lab Translational Oncology; Cellular disease models 1; Regenerative Medicine and Stem Cells; Cancer; Cluster B; Infection & Immunity; SCT patientenzorg; CMM Groep Coffer |
| Publication Year: | 2026 |
| Subject Terms: | General Neuroscience; General Immunology and Microbiology; General Biochemistry,Genetics and Molecular Biology |
| Description: | Mesenchymal stem/stromal cells (MSCs) are known for their regenerative properties. This protocol describes a co-culture system for investigating molecular interactions between MSCs and intestinal epithelial organoids following injury. We outline steps for assessing the immediate effects of MSCs on organoid growth and survival, as well as a model for evaluating longer term responses. The workflow is adaptable and can be readily modified to examine MSC interactions with additional cell types or in different injury contexts. For complete information on the use and execution of this protocol, please refer to Yetkin-Arik et al. |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 2666-1667 |
| Relation: | https://dspace.library.uu.nl/handle/1874/468946 |
| Availability: | https://dspace.library.uu.nl/handle/1874/468946 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| Accession Number: | edsbas.F163EA93 |
| Database: | BASE |