| Title: |
Action of Humicola lanuginosa lipase on mixed monomolecular films of tricaprylin and polyethylene glycol stearate |
| Authors: |
Ivanova, Tzvetanka; Mircheva, Kristina; Dobreva, G.; Panaiotov, Ivan; Proust, J.; Verger, R. |
| Contributors: |
Lomonosov Moscow State University = Université d'État Lomonossov de Moscou (MSU); Sofia University Saint Kliment Ohridski - Софийски университет „Свети Климент Охридски; Micro et Nanomédecines Biomimétiques (MINT); Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL); Institut de biologie et chimie des protéines Lyon (IBCP); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 0927-7765 ; EISSN: 1873-4367. |
| Publisher Information: |
CCSD; Elsevier |
| Publication Year: |
2008 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
Octanoic Acids/chemistry; Polyethylene Glycols/chemistry; Surface Properties; Triglycerides/chemistry; Chemical; Models; Lipase/chemistry; Kinetics; Hydrolysis; Hydrogen-Ion Concentration; Catalysis; Ascomycota/enzymology; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; The hydrolysis catalyzed by Humicola lanuginosa lipase (HLL) of pure tricaprylin (TC) or stearate of polyethylene glycol 1500 (PEG-St) as well as their mixtures spread as monomolecular films were studied. The catalytic transformation of the two substrates TC or PEG-St into their respective reaction products was detected by measuring simultaneously the decrease in the film area and the surface potential using the "zero order" trough at constant surface pressure. A kinetic model describing the enzymatic hydrolysis was developed. The surface concentrations of the two substrates and their respective reaction products as well as the values of the global kinetic constants of hydrolysis were determined. The experimentally obtained global kinetic constants of the catalytic action of HLL against TC and PEG-St present in mixed monolayers of TC/PEG-St are approximately the same as in the case of pure monolayers. These obtained results give some indications that the activity of enzyme is not significantly affected by the different molecular environments in the mixed monolayers. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
OKINA: ua3771 |
| Availability: |
https://univ-angers.hal.science/hal-03171788 |
| Accession Number: |
edsbas.F191ECA8 |
| Database: |
BASE |