| Title: |
Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People |
| Authors: |
Demaret, Julie; Corroyer-Simovic, Bénédicte; Alidjinou, E. K.; Goffard, Anne; Trauet, Jacques; Miczek, S.; Vuotto, F.; Dendooven, Arnaud; Huvent-Grelle, D.; Podvin, J.; Dreuil, D.; Faure, Karine; Deplanque, Dominique; Bocket, L.; Duhamel, A.; Labreuche, J.; Sobaszek, A.; Hisbergues, M.; Puisieux, F.; Labalette, Myriam; Lefevre, Guillaume |
| Contributors: |
Institute for Translational Research in Inflammation - U 1286 (INFINITE); Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille); Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille); Hôpital Gériatrique Les Bateliers Lille (USLD - Nord); Laboratoire de Virologie - ULR 3610 (Laboratoire de Virologie); Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille); Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL); Institut Pasteur de Lille; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS); Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS); Centre d'Investigation Clinique - Innovation Technologique de Lille - CIC 1403 - CIC 9301 (CIC Lille); Santé Publique : épidémiologie et qualité des soins (EA 2694); Faculté de Médecine Henri Warembourg - Université de Lille-Centre d'Etudes et de Recherche en Informatique Médicale Lille (CERIM); Université de Lille; ANR-20-COVI-0062,proteoCOVID,Protéomique clinique de la protéine SARS-CoV-2 Spike pour optimiser sa détection et le développement de tests sérologiques(2020) |
| Source: |
ISSN: 1664-3224. |
| Publisher Information: |
CCSD; Frontiers |
| Publication Year: |
2021 |
| Collection: |
LillOA (HAL Lille Open Archive, Université de Lille) |
| Subject Terms: |
SARS - CoV - 2; vaccine; older people and ageing; T cells response; mRNA vaccination; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Long-term care facility (LTCF) older residents display physiological alterations of cellular and humoral immunity that affect vaccine responses. Preliminary reports suggested a low early postvaccination antibody response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to focus on the specific T-cell response. We quantified S1-specific IgG, neutralizing antibody titers, total specific IFNγ-secreting T cells by ELISpot, and functionality of CD4+- and CD8+-specific T cells by flow cytometry, after two doses of the BNT162b2 vaccine in younger and older people, with and without previous COVID-19 infection (hereafter referred to as COVID-19-recovered and COVID-19-naive subjects, respectively). Frailty, nutritional, and immunosenescence parameters were collected at baseline in COVID-19-naive older people. We analyzed the immune response in 129 young adults (median age 44.0 years) and 105 older residents living in a LCTF (median age 86.5 years), 3 months after the first injection. Humoral and cellular memory responses were dramatically impaired in the COVID-19-naive older (n = 54) compared with the COVID-19-naive younger adults (n = 121). Notably, older participants’ neutralizing antibodies were 10 times lower than the younger’s antibody titers (p < 0.0001) and LCTF residents also had an impaired functional T-cell response: the frequencies of IFNγ+ and IFNγ+IL-2+TNFα+ cells among specific CD4+ T cells, and the frequency of specific CD8+ T cells were lower in COVID-19-naive older participants than in COVID-19-naive young adults (p < 0.0001 and p=0.0018, respectively). However, COVID-19-recovered older participants (n = 51) had greater antibody and T-cell responses, including IFNγ+ and IFNγ+IL-2+TNFα+-specific CD4+ T cells (p < 0.0001), as well as TNFα+-specific CD8+ T cells (p < 0.001), than COVID-19-naive older adults. We also observed that “inflammageing” and particularly high plasma levels of TNFα was associated to poor antibody ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34868051; PUBMED: 34868051; PUBMEDCENTRAL: PMC8637126 |
| DOI: |
10.3389/fimmu.2021.778679 |
| Availability: |
https://hal.univ-lille.fr/hal-03843676; https://hal.univ-lille.fr/hal-03843676v1/document; https://hal.univ-lille.fr/hal-03843676v1/file/Demaret%20et%20al.%202021.pdf; https://doi.org/10.3389/fimmu.2021.778679 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.F19723DE |
| Database: |
BASE |