| Title: |
ESCRT-III-dependent adhesive and mechanical changes are triggered by a mechanism detecting alteration of Septate Junction integrity in Drosophila epithelial cells |
| Authors: |
de Bournonville, Thomas Esmangart; Jaglarz, Mariusz, K.; Durel, Emeline; Le Borgne, Roland |
| Contributors: |
Ecole Polytechnique Fédérale de Lausanne (EPFL); Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ); Institut de Génétique et Développement de Rennes (IGDR); Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); Fondation pour la Recherche Médicale (FDT202001010770)Agence Nationale de la Recherche (ANR-20-CE13-0015)Fondation ARC pour la Recherche sur le Cancer (PJA 20191209388); ANR-20-CE13-0015,ACTRICE,Contribution des Jonctions Tricellulaires dans la Mécanique Epithéliale(2020) |
| Source: |
EISSN: 2050-084X ; eLife ; https://hal.science/hal-04444551 ; eLife, 2024, Elife, 13, pp.e91246. ⟨10.7554/eLife.91246⟩ |
| Publisher Information: |
HAL CCSD; eLife Sciences Publication |
| Publication Year: |
2024 |
| Collection: |
Université de Rennes 1: Publications scientifiques (HAL) |
| Subject Terms: |
D. melanogaster; developmental biology; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Barrier functions of proliferative epithelia are constantly challenged by mechanical and chemical constraints. How epithelia respond to and cope with disturbances of barrier functions to allow tissue integrity maintenance is poorly characterized. Cellular junctions play an important role in this process and intracellular traffic contribute to their homeostasis. Here, we reveal that, in Drosophila pupal notum, alteration of the bi- or tricellular septate junctions (SJs) triggers a mechanism with two prominent outcomes. On one hand, there is an increase in the levels of E-cadherin, F-Actin and non-muscle Myosin II in the plane of adherens junctions. On the other hand, β-integrin/Vinculin-positive cell contacts are reinforced along the lateral and basal membranes. We found that the weakening of SJ integrity, caused by the depletion of bi- or tricellular SJ components, alters ESCRT-III/Vps32/Shrub distribution, reduces degradation and instead favours recycling of SJ components, an effect that extends to other recycled transmembrane protein cargoes including Crumbs, its effector β-Heavy Spectrin Karst, and β-integrin. We propose a mechanism by which epithelial cells, upon sensing alterations of the septate junction, reroute the function of Shrub to adjust the balance of degradation/recycling of junctional cargoes and thereby compensate for barrier junction defects to maintain epithelial integrity. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/38305711; PUBMED: 38305711 |
| DOI: |
10.7554/eLife.91246 |
| Availability: |
https://hal.science/hal-04444551; https://hal.science/hal-04444551v1/document; https://hal.science/hal-04444551v1/file/elife-91246-v3.pdf; https://doi.org/10.7554/eLife.91246 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.F2C9B95 |
| Database: |
BASE |