| Title: |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| Authors: |
Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O'Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios-Gutiérrez, Juan-José; Tudó, Griselda; Bou, German; Ceyssens, Pieter-Jan; Garrigo, Montserrat; Gonzalez-Martin, Julian; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla-Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B.; Quiroga, Lidia; Ruiz-Serrano, Maria Jesús; Rodríguez-Sánchez, Belén; Universitat Autònoma de Barcelona |
| Publication Year: |
2022 |
| Collection: |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
| Subject Terms: |
Clinical microbiology; Infectious-disease diagnostics |
| Description: |
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
20452322 |
| Relation: |
Ministerio de Economía y Competitividad PI15/01073; Instituto de Salud Carlos III PI18/00997; Instituto de Salud Carlos III PI18/01068; Scientific reports; Vol. 12 Núm. 1 (december 2022), p. 1237; https://ddd.uab.cat/record/290571; urn:oai:ddd.uab.cat:290571; urn:scopus_id:85123571981; urn:articleid:20452322v12n1p1237; urn:pmid:35075208; urn:pmcid:PMC8786948; urn:oai:pubmedcentral.nih.gov:8786948 |
| Availability: |
https://ddd.uab.cat/record/290571 |
| Rights: |
open access ; Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.F329DF32 |
| Database: |
BASE |