| Title: |
Production of platelets in vitro in functionalized 3-dimensional scaffolds mimicking the bone marrow niche |
| Authors: |
Foster, Holly R; Colzani, Maria; Bouet, Guenaelle; Howard, Daniel; Di Buduo, Christian A; Müller-Sienerth, Nicole; Waller, Amie K; Sun, Yi; Davidenko, Natalia; Shepherd, Jennifer H; Moreau, Thomas; Evans, Amanda L; Soprano, Paolo M; Parsons, Martin EM; Sims, Yumi Ying; Arumugam, Meera; Afshar-Saber, Wardiya; Turro, Ernest; Maguire, Patricia B; Best, Serena M; Cameron, Ruth E; Balduini, Alessandra; Wright, Gavin J; Ghevaert, Cedric |
| Publisher Information: |
Ferrata Storti Foundation (Haematologica); Department of Haematology; Department of Materials Science and Metallurgy; //doi.org/10.3324/haematol.2024.286758 |
| Publication Year: |
2025 |
| Collection: |
Apollo - University of Cambridge Repository |
| Subject Terms: |
Humans; Blood Platelets; Megakaryocytes; Tissue Scaffolds; Thrombopoiesis; Stem Cell Niche; Fetal Blood; Bone Marrow; Cells; Cultured |
| Description: |
The safety, quality and supply of donor-derived platelet units intended for transfusion have improved over the past decades but significant problems still remain. In vitroderived platelets offer a possible alternative but up-scaling production is hindered by our limited understanding of thrombopoiesis (the release of platelets by their mother cell, the megakaryocyte [MK]). Here, we have developed an integrated strategy aiming to mimic ex vivo the bone marrow physiological niche that promotes thrombopoiesis by mature MKs. The screening of a panel of 259 recombinant transmembrane proteins derived from cells known to promote platelet production through direct contact with MKs enabled us to show that ACVR1B, CRTAM, MUCEN and BTN1A1 improve platelet production from either cord blood- (ACVR1B) or pluripotent stem cells-derived (CRTAM, MUCEN and BTN1A1) MKs. Using two different methodologies, we functionalise either collagen- or silkbased 3-dimensional scaffolds and confirm increased functional platelet production by up to two-fold. This unbiased approach has allowed us to identify novel proteins whose role in platelet formation was previously unknown and highlights the potential gain of recreating the MK niche to allow in vitro platelets to become a viable alternative for transfusion. ; NHS Blood and Transplant (WP15-06) (HRF. and MA) Medical Research Council Centre (MR/L022982/1) (GBC.) National Institute for Health Research (NIHR; RP-PG-0310-1002) (MC) Wellcome Trust (206194) Science Foundation Ireland (19/FIP/AI/7490) (PBM) EU Horizon 2020 (767309) (DH and AKW) European Research Council (ERC) (320598 3D-E) (SMB and REC) NHS Blood and Transplant (CG) European Commission Grant (H2020-FETOPEN-1-2016-2017-SilkFusion, Grant Agreement 767309) (AB and CG) US National Institutes of Health (R01 EB016041-02) (AB and CG) |
| Document Type: |
article in journal/newspaper |
| File Description: |
Print-Electronic; application/pdf |
| Language: |
English |
| Relation: |
https://www.repository.cam.ac.uk/handle/1810/396000 |
| Availability: |
https://www.repository.cam.ac.uk/handle/1810/396000 |
| Rights: |
Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.F34284C4 |
| Database: |
BASE |