| Title: |
Outer-Membrane-Vesicle-Associated O Antigen, a Crucial Component for Protecting Against Bordetella parapertussis Infection |
| Authors: |
Bottero, Daniela; Zurita, María Eugenia; Gaillard, María Emilia; Carriquiriborde, Francisco Pablo; Aispuro, Pablo Martín; Elizagaray, Maia Lina; Bartel, Erika Belén; Castuma, Celina Elisabet; Hozbor, Daniela Flavia |
| Publication Year: |
2018 |
| Collection: |
Universidad Nacional de La Plata (UNLP): SeDiCI (Servicio de Difusión de la Creación Intelectual) |
| Subject Terms: |
Ciencias Exactas; Biología; Bordetella parapertussis; lipopolysacharides; O-antigen; outer-membrane vesicles; protection |
| Description: |
Bordetella parapertussis is a respiratory-disease pathogen producing symptomatology similar to that of pertussis but of underestimated incidence and with no specific vaccine existing. We recently designed a vaccine candidate from B. parapertussis outer-membrane vesicles (OMVs) that proved to be safe and protective in a murine-infection model. Based on protection recently reported for the B. parapertussis O antigen in aqueous solution, we assessed here whether the B. parapertussis O-antigen-containing lipopolysaccharide (BppLPS-O⁺) embedded in the membranes, as present in B. parapertussis -derived OMVs (OMVs(Bpp-LPS-O⁺)), was the component responsible for that previously observed protection by OMVs. By performing a comparative study with OMVs from a human strain with undetectable O antigen (OMVs(Bpp-LPS-O⁻)), we demonstrated that the OMVs(Bpp-LPS-O⁺), but not the OMVs(Bpp-LPS-O⁻), protected mice against sublethal B. parapertussis infections. Indeed, the B. parapertussis loads were significantly reduced in the lungs of OMVs(Bpp-LPS-O⁺) -vaccinated animals, with the CFUs recovered being decreased by 4 log units below those detected in the non-immunized animals or in the animals treated with the OMVs(Bpp-LPS-O⁻), (p < 0.001). We detected that the OMVs(Bpp-LPS-O⁺) induced IgG antibodies against B. parapertussis whole-cell lysates, which immunocomponents recognized, among others, the O antigen and accordingly conferred protection against B. parapertussis infection, as observed in in-vivo -passive-transfer experiments. Of interest was that the OMVs(Bpp-LPS-O⁺) -generated sera had opsonophagocytic and bactericidal capabilities that were not detected with the OMVs(Bpp-LPS-O⁻)-induced sera, suggesting that those activities were involved in the clearance of B. parapertussis . Though stimulation of cultured spleen cells from immunized mice with formulations containing the O antigen resulted in gamma interferon (IFN-γ) and interleukin-17 production, spleen cells from OMVs(Bpp-LPS-O⁺) -immunized mice did not significantly ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
http://sedici.unlp.edu.ar/handle/10915/104549 |
| Availability: |
http://sedici.unlp.edu.ar/handle/10915/104549 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ ; Creative Commons Attribution 4.0 International (CC BY 4.0) |
| Accession Number: |
edsbas.F3781323 |
| Database: |
BASE |