| Title: |
Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury |
| Authors: |
Stüdemann, Tim; Rössinger, Judith; Manthey, Christoph; Geertz, Birgit; Srikantharajah, Rajiven; von Bibra, Constantin; Shibamiya, Aya; Köhne, Maria; Wiehler, Antonius; Wiegert, J. Simon; Eschenhagen, Thomas; Weinberger, Florian |
| Source: |
Circulation ; volume 146, issue 15, page 1159-1169 ; ISSN 0009-7322 1524-4539 |
| Publisher Information: |
Ovid Technologies (Wolters Kluwer Health) |
| Publication Year: |
2022 |
| Description: |
Background: Transplantation of pluripotent stem cell–derived cardiomyocytes represents a promising therapeutic strategy for cardiac regeneration, and the first clinical studies in patients with heart failure have commenced. Yet, little is known about the mechanism of action underlying graft-induced benefits. Here, we explored whether transplanted cardiomyocytes actively contribute to heart function. Methods: We injected cardiomyocytes with an optogenetic off-on switch in a guinea pig cardiac injury model. Results: Light-induced inhibition of engrafted cardiomyocyte contractility resulted in a rapid decrease of left ventricular function in ≈50% (7/13) animals that was fully reversible with the offset of photostimulation. Conclusions: Our optogenetic approach demonstrates that transplanted cardiomyocytes can actively participate in heart function, supporting the hypothesis that the delivery of new force-generating myocardium can serve as a regenerative therapeutic strategy. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1161/circulationaha.122.060124 |
| DOI: |
10.1161/CIRCULATIONAHA.122.060124 |
| Availability: |
https://doi.org/10.1161/circulationaha.122.060124; https://www.ahajournals.org/doi/full/10.1161/CIRCULATIONAHA.122.060124 |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Accession Number: |
edsbas.F504B16A |
| Database: |
BASE |