| Title: |
Risk Stratification of Prostate Cancer Through Quantitative Assessment of PTEN Loss (qPTEN) |
| Authors: |
Jamaspishvili, Tamara; Patel, Palak G; Niu, Yi; Vidotto, Thiago; Caven, Isabelle; Livergant, Rachel; Fu, Winnie; Kawashima, Atsunari; How, Nathan; Okello, John B; Guedes, Liana B; Ouellet, Veronique; Picanço, Clarissa; Koti, Madhuri; Reis, Rodolfo B; Saad, Fred; Mes-Masson, Anne-Marie; Lotan, Tamara L; Squire, Jeremy A; Peng, Yingwei P; Siemens, D Robert; Berman, David M |
| Contributors: |
Prostate Cancer Canada; Movember Foundation; Transformative Pathology Fellowship; Ontario Institute for Cancer Research; Terry Fox Transdisciplinary Fellowship; FAPESP; CNPq Bolsa Produtividade em Pesquisa - Nível: PQ-1B; Terry Fox Research Institute-Canadian Prostate Cancer Biomarker Network; Canadian Institutes for Health Research |
| Source: |
JNCI: Journal of the National Cancer Institute ; volume 112, issue 11, page 1098-1104 ; ISSN 0027-8874 1460-2105 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2020 |
| Description: |
Background Phosphatase and tensin homolog (PTEN) loss has long been associated with adverse findings in early prostate cancer. Studies to date have yet to employ quantitative methods (qPTEN) for measuring of prognostically relevant amounts of PTEN loss in postsurgical settings and demonstrate its clinical application. Methods PTEN protein levels were measured by immunohistochemistry in radical prostatectomy samples from training (n = 410) and validation (n = 272) cohorts. PTEN loss was quantified per cancer cell and per tissue microarray core. Thresholds for identifying clinically relevant PTEN loss were determined using log-rank statistics in the training cohort. Univariate (Kaplan-Meier) and multivariate (Cox proportional hazards) analyses on various subpopulations were performed to assess biochemical recurrence-free survival (BRFS) and were independently validated. All statistical tests were two-sided. Results PTEN loss in more than 65% cancer cells was most clinically relevant and had statistically significant association with reduced BRFS in training (hazard ratio [HR] = 2.48, 95% confidence interval [CI] = 1.59 to 3.87; P < .001) and validation cohorts (HR = 4.22, 95% CI = 2.01 to 8.83; P < .001). The qPTEN scoring method identified patients who recurred within 5.4 years after surgery (P < .001). In men with favorable risk of biochemical recurrence (Cancer of the Prostate Risk Assessment – Postsurgical scores |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/jnci/djaa032 |
| DOI: |
10.1093/jnci/djaa032/33210617/djaa032.pdf |
| Availability: |
https://doi.org/10.1093/jnci/djaa032; http://academic.oup.com/jnci/advance-article-pdf/doi/10.1093/jnci/djaa032/33210617/djaa032.pdf; http://academic.oup.com/jnci/article-pdf/112/11/1098/34333281/djaa032.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.F5FF87F8 |
| Database: |
BASE |