| Title: |
VelcroVax: a ‘bolt-on’ vaccine platform for glycoprotein display |
| Authors: |
Kingston, NJ; Grehan, K; Snowden, JS; Hassall, M; Alzahrani, J; Paesen, GC; Sherry, L; Hayward, C; Roe, A; Stephen, S; Tomlinson, D; Zeltina, A; Doores, KJ; Ranson, NA; Stacey, M; Page, M; Rose, NJ; Bowden, TA; Rowlands, DJ; Stonehouse, N |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2023 |
| Collection: |
White Rose Research Online (Universities of Leeds, Sheffield & York) |
| Description: |
Having varied approaches to the design and manufacture of vaccines is critical in being able to respond to worldwide needs and newly emerging pathogens. Virus-like particles (VLPs) form the basis of two of the most successful licensed vaccines (against hepatitis B virus [HBV] and human papillomavirus). They are produced by recombinant expression of viral structural proteins, which assemble into immunogenic nanoparticles. VLPs can be modified to present unrelated antigens, and here we describe a universal "bolt-on" platform (termed VelcroVax) where the capturing VLP and the target antigen are produced separately. We utilize a modified HBV core (HBcAg) VLP with surface expression of a high-affinity binding sequence (Affimer) directed against a SUMO tag and use this to capture SUMO-tagged gp1 glycoprotein from the arenavirus Junín virus (JUNV). Using this model system, we have solved the first high-resolution structures of VelcroVax VLPs and shown that the VelcroVax-JUNV gp1 complex induces superior humoral immune responses compared to the noncomplexed viral protein. We propose that this system could be modified to present a range of antigens and therefore form the foundation of future rapid-response vaccination strategies. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
2379-5042 |
| Relation: |
https://eprints.whiterose.ac.uk/id/eprint/194460/19/VelcroVax%20a%20%E2%80%98bolt-on%E2%80%99%20vaccine%20platform%20for%20glycoprotein%20.pdf; Kingston, NJ, Grehan, K orcid.org/0000-0003-3976-756X , Snowden, JS et al. (17 more authors) (2023) VelcroVax: a ‘bolt-on’ vaccine platform for glycoprotein display. mSphere, 8 (1). e0056822. ISSN: 2379-5042 |
| Availability: |
https://eprints.whiterose.ac.uk/id/eprint/194460/ |
| Rights: |
cc_by_4 |
| Accession Number: |
edsbas.F7436890 |
| Database: |
BASE |