| Title: |
Immunomodulatory Effects of Endoscopic Ultrasound-Guided Thermal Ablation in Patients with Pancreatic Ductal Adenocarcinoma |
| Authors: |
Testoni S. G. G.; Minici C.; Benetti E.; Clemente F.; Boselli D.; Sciorati C.; De Monte L.; Petrone M. C.; Enderle M.; Linzenbold W.; Protti M. P.; Manfredi A.; De Cobelli F.; Reni M.; Falconi M.; Capurso G.; Arcidiacono P. G.; Della-Torre E. |
| Contributors: |
Testoni, S. G. G.; Minici, C.; Benetti, E.; Clemente, F.; Boselli, D.; Sciorati, C.; De Monte, L.; Petrone, M. C.; Enderle, M.; Linzenbold, W.; Protti, M. P.; Manfredi, A.; De Cobelli, F.; Reni, M.; Falconi, M.; Capurso, G.; Arcidiacono, P. G.; Della-Torre, E. |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute (MDPI) |
| Publication Year: |
2023 |
| Subject Terms: |
ablation techniques; B Lymphocytes; cytokines; immunomodulation; monocytes; pancreatic cancer; T Lymphocytes |
| Description: |
Immunological consequences of endoscopic ultrasound (EUS)-local thermal ablation (LTA) for pancreatic ductal adenocarcinoma (PDAC) have not been extensively assessed. We aimed to explore EUS-LTA effects on the systemic immune response in PDAC. Peripheral blood was collected from 10 treatment-naïve patients with borderline resectable and locally advanced PDAC, randomly allocated to Nab-paclitaxel plus Gemcitabine chemotherapy (CT-arm, n = 5) or EUS-LTA with HybridTherm Probe plus CT (HTP + CT-arm, n = 5). Twenty healthy donors were included as controls. Flow-cytometry and multiplex assays were used to profile immune cell subsets and measure serum cytokines/chemokines, respectively. At baseline, PDAC patients showed increased circulating monocytes and lower circulating lymphocytes and CD19+ B cells counts compared to healthy controls. After 4 months, CT induced decrease of B regulatory cells, CD4+ cytotoxic T cells and IL-1β. The addition of EUS-HTP to CT selectively decreased the serum levels of APRIL/TNFSF13 as well as T regulatory cells, total, classic and inflammatory monocytes. Serum levels of APRIL/TNFSF13 and total, classic and inflammatory monocytes counts at baseline were associated with worse overall survival. EUS-HTP has the potential to selectively impact on immune cells and cytokines associated with poor outcomes in PDAC. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/37509365; info:eu-repo/semantics/altIdentifier/wos/WOS:001035110400001; volume:15; issue:14; firstpage:3704; journal:CANCERS; https://hdl.handle.net/20.500.11768/149778 |
| DOI: |
10.3390/cancers15143704 |
| Availability: |
https://hdl.handle.net/20.500.11768/149778; https://doi.org/10.3390/cancers15143704; https://www.mdpi.com/2072-6694/15/14/3704 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.F7F7FD0B |
| Database: |
BASE |