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A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues

Title: A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues
Authors: Steele, L; Olabi, B; Roberts, K; Mazin, PV; Koplev, S; Tudor, C; Rumney, B; Admane, C; Jiang, T; Correa-Gallegos, D; Chakala, KP; Binkevich, A; Gopee, NH; Predeus, A; Prete, M; Winheim, E; Annusver, K; Forsthuber, A; Francis, L; Frech, S; Ganier, C; Layton, T; Liu, Y; Yuan, H; Gudjonsson, JE; Lichtenberger, BM; Mahil, S; Nanchahal, J; O'Toole, EA; Plikus, MV; Rinkevich, Y; Rognoni, E; Smith, CH; Teichmann, SA; Kasper, M; Foster, AR; Lotfollahi, M; Haniffa, M
Publisher Information: Nature Research
Publication Year: 2025
Collection: Oxford University Research Archive (ORA)
Description: Fibroblasts sculpt the architecture and cellular microenvironments of various tissues. Here we constructed a spatially resolved atlas of human skin fibroblasts from healthy skin and 23 skin diseases, with comparison to 14 cross-tissue diseases. We define six major skin fibroblast subtypes in health and three that are disease-specific. We characterize two fibroblast subtypes further as they are conserved across tissues and are immune-related. The first, F3: fibroblastic reticular cell-like fibroblast (CCL19+CD74+HLA-DRA+), is a fibroblastic reticular cell-like subtype that is predicted to maintain the superficial perivascular immune niche. The second, F6: inflammatory myofibroblasts (IL11+MMP1+CXCL8+IL7R+), characterizes early human skin wounds, inflammatory diseases with scarring risk and cancer. F6: inflammatory myofibroblasts were predicted to recruit neutrophils, monocytes and B cells across multiple human tissues. Our study provides a harmonized nomenclature for skin fibroblasts in health and disease, contextualized with cross-tissue findings and clinical skin disease profiles.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1038/s41590-025-02267-8
Availability: https://doi.org/10.1038/s41590-025-02267-8; https://ora.ox.ac.uk/objects/uuid:e84042a2-aa15-4e92-bbd8-f279575293b6
Rights: info:eu-repo/semantics/openAccess ; CC Attribution (CC BY)
Accession Number: edsbas.F7FD0E47
Database: BASE