| Title: |
Expression, crystallization and preliminary crystallographic study of GluB from Corynebacterium glutamicum |
| Authors: |
Crystallization; Qingbo Liu; B Defeng Li; Yonglin Hua |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
http://library.ibp.ac.cn/html/slwj/000319736000015.pdf. |
| Publication Year: |
2013 |
| Collection: |
CiteSeerX |
| Description: |
GluB is a substrate-binding protein (SBP) which participates in the uptake of glutamic acid in Corynebacterium glutamicum, a Gram-positive bacterium. It is part of an ATP-binding cassette (ABC) transporter system. Together with the transmembrane proteins GluC and GluD and the cytoplasmic protein GluA, which couples the hydrolysis of ATP to the translocation of glutamate, they form a highly active glutamate-uptake system. As part of efforts to study the amino-acid metabolism, especially the metabolism of glutamic acid by C. glutamicum, a bacterium that is widely used in the industrial production of glutamic acid, the GluB protein was expressed, purified and crystallized, an X-ray diffraction data set was collected to a resolution of 1.9 A ̊ and preliminary crystallographic analysis was performed. The crystal belonged to space group P3121 or P3221, with unit-cell parameters a = b = 82.50, c = 72.69 Å. 1. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| ISBN: |
978-0-00-319736-5; 0-00-319736-0; 978-0-00-319736-5; 0-00-319736-0 |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.661.8547; http://library.ibp.ac.cn/html/slwj/000319736000015.pdf |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.661.8547; http://library.ibp.ac.cn/html/slwj/000319736000015.pdf |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.F8D0BD5D |
| Database: |
BASE |