| Title: |
Calciprotein crystallization time (T50) and its association with surrogate cardiovascular disease risk markers in individuals with type 2 diabetes mellitus:the cross-sectional EARLY-HFpEF study. |
| Authors: |
Meer, R; Hoek,A G; Dal Canto, E; Doesburg,T; Pasch,A; Vervloet,M G; de Jong, P A; Elders,P J M; Beulens, J W J; Experimentele Afd. Cardiologie 1; Circulatory Health; Cancer; Infection & Immunity; Regenerative Medicine and Stem Cells; JC onderzoeksprogramma Cardiovascular Health |
| Publication Year: |
2026 |
| Subject Terms: |
Journal Article |
| Description: |
BACKGROUND: Heart failure and peripheral artery disease (PAD) are the two most common cardiovascular diseases (CVD) in individuals with type 2 diabetes mellitus (T2DM). The T 50 calciprotein crystallization test measures the transformation of calciprotein particles type 1 (CPP1) into CPP2 in vitro and has been introduced as a low-cost biomarker for arterial calcification and CVD risk. We aimed to investigate the association between T 50 and (1) heart failure with preserved ejection fraction (HFpEF), (2) ankle-brachial index (ABI) as measure of PAD, (3) pulse wave velocity (PWV) as measure of central arterial stiffness and (4) arterial calcification in individuals with T2DM. METHODS: Cross-sectional data was used of 771 individuals with T2DM (64% men, 67 [63-71] years). T 50 was measured using nephelometry on non-fasting serum samples. Presence of HFpEF was assessed with echocardiography based on current guidelines. ABI was categorized as ≤ 0.9 (PAD), 0.9-1.4 (normal) and ≥ 1.4 (high). Central arterial stiffness was measured using carotid-femoral PWV. Lower-extremity and coronary calcification were measured using computed tomography and quantified using Agatston scores categorized into zero (reference category) and tertiles > 0. Multivariable-adjusted Poisson, multinomial and linear regression analyses were used to study the associations with aforementioned surrogate CVD risk markers. RESULTS: Mean T 50 was 355 ± 55 min. HFpEF and PAD were present in 36.6% and 5.8% of the cohort, respectively. Mean cfPWV was 12.9 ± 2.5 m/s. Median calcification scores in the coronary arteries and lower-extremities were 315 [40-1246] and 791 [64-3820] Agatston units, respectively. Every 60-min decrease in T 50, indicating higher calcification risk, was associated with increased coronary arterial calcification (e.g. highest tertile OR = 1.63 [1.15-2.30], p = 0.006), but not with lower-extremity arterial calcification (e.g. highest tertile OR = 1.28 [0.96-1.69], p = 0.088). Moreover, T 50 ≤ 330 min versus T 50 ≥ 390 min was ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1475-2840 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/469120 |
| Availability: |
https://dspace.library.uu.nl/handle/1874/469120 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.F8F5DEFD |
| Database: |
BASE |