| Title: |
Expression of ERAlfa, its ERAlfaTrangle3 Splice Variant and Gama-SYNUCLEIN in Ovarian Cancer: A Pilot Study |
| Authors: |
Cheung, Karen T.; Taylor, Siân E.; Patel, Imran I.; Bentley, Adam J.; Stringfellow, Helen F.; Fullwood, Nigel J.; Martin-Hirsch, Pierre L.; Martin, Francis L. |
| Publisher Information: |
Zenodo |
| Publication Year: |
2011 |
| Collection: |
Zenodo |
| Subject Terms: |
Endothelial cells; ovarian cancer; ERa; ERaΔ3; g-SYNUCLEIN; neoangiogenesis |
| Description: |
Aims: Ovarian cancer has the highest mortality of any gynaecological malignancy; this is due to rapid peritoneal spread of tumour cells and neovascularization. Understanding the mechanisms underlying this is critical to developing early diagnostic or treatment strategies. We devised a pilot study to examine the role of g-SYNUCLEIN (g-SYN), oestrogen receptor (ER)a, and the splice variant ERaΔ3. Methodology: With ethical approval, ovarian tissue was collected from patients (n=24) undergoing oopherectomy for non-ovarian pathology or primary surgery for suspected ovarian cancer. Quantitative gene expression analysis was employed for g-SYN, ERa, and ERaΔ3. To identify the in situ localization, immunofluorescence for g-syn was carried out. Results: Ovarian tumour tissue exhibited an elevated expression of g-SYN and high-grade tumours had an elevated ERaΔ3:ERa ratio compared with benign tissue. The majority of previous studies point to the g-syn protein being present in epithelial cells of high-grade disease. Our study supports this, but additionally we conclusively identify its presence in the endothelial cells of vasculature surrounding low-grade disease; immunofluorescence was strongest in the apical cells surrounding the lumen. Conclusion: Our results demonstrate for the first time that there are readily-expressed levels of g-SYN and ERaΔ3 in normal ovarian tissue and ovarian tumours. In high-grade disease, g-syn and an elevated ERaΔ3:ERa ratio might confer metastatic potential to the tumourigenic cells and promote neoangiogenesis. Future in vitro studies might be necessary to delineate such a mechanism, which could potentially be the basis of early intervention. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://zenodo.org/communities/2249-0205/; https://zenodo.org/records/7802; oai:zenodo.org:7802; https://doi.org/10.5281/zenodo.7802 |
| DOI: |
10.5281/zenodo.7802 |
| Availability: |
https://doi.org/10.5281/zenodo.7802; https://zenodo.org/records/7802 |
| Rights: |
Creative Commons Attribution 4.0 International ; cc-by-4.0 ; https://creativecommons.org/licenses/by/4.0/legalcode |
| Accession Number: |
edsbas.FA195EC0 |
| Database: |
BASE |