| Title: |
Polythionine and gold nanostar-based impedimetric aptasensor for label-free detection of α-synuclein oligomers |
| Authors: |
Tao, Dan; Wang, Jiao; Song, Shizhen; Cai, Ke; Jiang, Mingdi; Cheng, Jing; Hu, Liang; Jaffrezic-Renault, Nicole; Guo, Zhenzhong; Pan, Hongzhi |
| Contributors: |
Hubei Prov Key Lab Occupat Hazard Identificat & C; Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan (Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan); Sch Publ Hlth, Med Coll; Wuhan University of Science & Technology; Resources & Environm Engn Coll; State Key Lab Radiat Med & Protect; Suzhou Vocational University; ISA-Micro & Nanobiotechnologies; Institut des Sciences Analytiques (ISA); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); Shanghai Univ Med & Hlth Sci; This work was supported by the National Natural Science Foundation of China (Grant Numbers 81973097 and 51873137), Hubei Province Introduction of Foreign Talents Project, China (Grant Number WGZJ2020000046). |
| Source: |
ISSN: 0021-891X. |
| Publisher Information: |
CCSD; Springer Verlag |
| Publication Year: |
2021 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
Au nanostars; Polythionine; Electrochemical impedance spectroscopy; alpha-Synuclein oligomers; Parkinson's disease; [CHIM.ANAL]Chemical Sciences/Analytical chemistry; [CHIM]Chemical Sciences; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; The neurotoxicity of alpha-synuclein oligomers (alpha-syno) could cause Parkinson's disease (PD) which is an irreversible neurodegenerative disease. In order to diagnose and treat PD patients at an early stage and reduce the damage of sampling to humans, a non-invasive measure is used to analyze ultra-trace levels of alpha-syno. In this work, a new impedimetric aptasensor based polythionine (pTH)/Au nanostars (AuNSs) platform was designed for label-free detection of alpha-syno. The pTH and AuNSs demonstrated excellent electrical conductivity, fast electron transfer capability, and the synergistic amplification effect, which could enhance the sensitivity and improve the limit of detection (LOD). The ssDNA aptamers recognized and bound to alpha-syno via specific bases. Therefore, the ultra-sensitive alpha-syno aptasensor based on pTH and AuNSs presented a LOD of 0.07 aM and a dynamic range from 0.10 aM to 10.00 fM. The aptasensor also displayed good response range, high sensitivity, and low LOD in diluted human plasma samples to provide new methods for early diagnosis of PD. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1007/s10800-021-01589-3 |
| Availability: |
https://hal.science/hal-03267496; https://doi.org/10.1007/s10800-021-01589-3 |
| Accession Number: |
edsbas.FAB2335D |
| Database: |
BASE |