| Title: |
Synthesis of rhamnosylated arginine glycopeptides and determination of the glycosidic linkage in bacterial elongation factor P |
| Authors: |
Wang, S; Corcilius, L; Sharp, PP; Rajkovic, A; Ibba, M; Parker, BL; Payne, RJ |
| Publisher Information: |
ROYAL SOC CHEMISTRY |
| Publication Year: |
2017 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
A new class of N-linked protein glycosylation - arginine rhamnosylation - has recently been discovered as a critical modification for the function of bacterial elongation factor P (EF-P). Herein, we describe the synthesis of suitably protected α- and β-rhamnosylated arginine amino acid "cassettes" that can be directly installed into rhamnosylated peptides. Preparation of a proteolytic fragment of Pseudomonas aeruginosa EF-P bearing both α- and β-rhamnosylated arginine enabled the unequivocal determination of the native glycosidic linkage to be α through 2D NMR and nano-UHPLC-tandem mass spectrometry studies. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
2041-6520 |
| Relation: |
pii: c6sc03847f; https://hdl.handle.net/11343/251057 |
| Availability: |
https://hdl.handle.net/11343/251057 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.FD2F8EBA |
| Database: |
BASE |