| Title: |
Establishment of a colony of Anopheles darlingi from French Guiana for vector competence studies on malaria transmission |
| Authors: |
Puchot, Nicolas; Lecoq, Marie-Thérèse; Carinci, Romuald; Duchemin, Jean Bernard; Gendrin, Mathilde; Bourgouin, Catherine |
| Contributors: |
Génétique fonctionnelle des maladies infectieuses - Functional Genetics of Infectious Diseases; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité); Institut Pasteur de la Guyane; Pasteur Network (Réseau International des Instituts Pasteur); Département Parasites et Insectes vecteurs - Department of Parasites and Insect Vectors; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); This work was supported by funds from the LabEx IBEID (The Laboratory of Excellence (LabEx) Integrative Biology of Emerging Infectious Diseases—grant no. ANR-10-LABX-62-IBEID) to CB and MG, FEDER-Contrôle to JD and RC, ANR JCJC MosMi to MG (grant no. ANR-18-CE15-0007), and a Calmette-Yersin Fellowship from Pasteur Network to RC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank members of the CEPIA platform for infecting our mosquitoes (An. darlingi and An. stephensi) with P. berghei and P. falciparum. Thanks to current and previous members of the Vectopole, especially Pascal Gaborit for technical help and to Anavaj Sakuntabhai for his constant support.; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010); ANR-18-CE15-0007,MosMi,Remodelage du microbiote de moustique pour étudier son impact sur la transmission du paludisme(2018); European Project: FEDER-Contrôle |
| Source: |
ISSN: 2673-7515 ; Frontiers in Tropical Diseases ; https://hal.science/hal-03960688 ; Frontiers in Tropical Diseases, 2022, 3, pp.949300. ⟨10.3389/fitd.2022.949300⟩. |
| Publisher Information: |
CCSD |
| Publication Year: |
2022 |
| Subject Terms: |
Anopheles darlingi colony rearing Plasmodium susceptibility French Guiana; Anopheles darlingi; colony; rearing; Plasmodium; susceptibility; French Guiana; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology |
| Description: |
Sec. Vector Biology ; International audience ; Anopheles darlingi is a major vector of both Plasmodium falciparum and Plasmodium vivax in South and Central America including French Guiana. However, the vector competence and physiology of this mosquito species have been scarcely studied due to difficulties in rearing it in the laboratory. Here, we report the successful establishment of a robust colony, from a mosquito collection in French Guiana. We describe our mosquito colonization procedure with relevant information on environmental conditions, mating ability, larval development, and survival, recorded over the first six critical generations. Experimental infection showed that our An. darlingi colony has a moderate permissiveness to in vitro produced gametocytes of the P. falciparum NF54 strain originating from Africa. This colony, which has reached its 21st generation, will allow further characterization of An. darlingi life-history traits and of Plasmodium –mosquito interactions with South American malaria parasites. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.3389/fitd.2022.949300 |
| Availability: |
https://hal.science/hal-03960688; https://hal.science/hal-03960688v1/document; https://hal.science/hal-03960688v1/file/fitd-03-949300.pdf; https://doi.org/10.3389/fitd.2022.949300 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.FDAB3AB0 |
| Database: |
BASE |